Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tyramide signal amplification integrated with magnetic bead separation to overcome the matrix effect and detect SAA protein in peripheral blood.

The Analyst·2026
Same author

High-Throughput Virtual Screening of Small Molecule Quenchers for Near-Infrared Fluorophores.

Angewandte Chemie (International ed. in English)·2026
Same author

Sensight enables quantitative multivariate engineering of high-performance chemical imaging tools.

Nature communications·2026
Same author

Precision Design of Fluorogenic Probes via Orthogonal Tuning of Binding and Photophysics for Isoform-Selective ALDH2 Imaging.

Journal of the American Chemical Society·2025
Same author

Detecting ALDH2 activity in live cells <i>via</i> conditional metabolic labeling.

Chemical science·2025
Same author

Organelle-resolved imaging of formaldehyde reveals its spatiotemporal dynamics.

Journal of materials chemistry. B·2024

Related Experiment Video

Updated: Jun 27, 2025

Application of Genetically Encoded Fluorescent Nitric Oxide (NO&#8226;) Probes, the geNOps, for Real-time Imaging of NO&#8226; Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

12.8K

Deconvoluting nitric oxide-protein interactions with spatially resolved multiplex imaging.

Yi Li1, Kaijun Pan2, Yanan Gao2

  • 1College of Pharmaceutical Sciences, National Key Laboratory of Advanced Drug Delivery and Release Systems, Zhejiang University 866 Yuhangtang Street Hangzhou 310058 China lixin81@zju.edu.cn.

Chemical Science
|May 3, 2024
PubMed
Summary

A new probe, NOP-1, enables simultaneous imaging of nitric oxide (NO) and proteins. This tool reveals NO’s role in hypoglycemia-induced brain injury by permanently staining proteins, aiding in understanding NO-protein interactions.

More Related Videos

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.9K
Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.4K

Related Experiment Videos

Last Updated: Jun 27, 2025

Application of Genetically Encoded Fluorescent Nitric Oxide (NO&#8226;) Probes, the geNOps, for Real-time Imaging of NO&#8226; Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

12.8K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.9K
Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.4K

Area of Science:

  • Chemical Biology
  • Neuroscience
  • Biomedical Imaging

Background:

  • Simultaneous imaging of nitric oxide (NO) and its interacting proteins is crucial for understanding NO-protein dynamics.
  • Existing NO imaging probes lack compatibility with immunostaining, limiting their application in complex biological samples.
  • There is a need for novel probes that can provide a permanent record of NO signaling events for subsequent analysis.

Purpose of the Study:

  • To develop a novel imaging probe (NOP-1) capable of detecting nitric oxide (NO) and labeling proximal proteins simultaneously.
  • To create a fluorogenic probe that translates transient NO signals into a permanent, immunostaining-compatible stain.
  • To investigate the role of NO in hypoglycemia-induced neurological injury using the developed probe.

Main Methods:

  • Development of NOP-1 by fusing acyl benzotriazole chemistry with a Si-rhodamine fluorophore for NO-triggered protein conjugation and fluorescence.
  • Utilizing NOP-1 for simultaneous imaging of NO signaling and protein localization in cellular models.
  • Performing multiplex imaging to correlate NOP-1 fluorescence with immunofluorescence markers like α-tubulin and nitrotyrosine (NO2-Tyr).

Main Results:

  • NOP-1 was successfully synthesized, exhibiting a non-fluorescent state that becomes fluorogenic upon NO-triggered conjugation to proximal proteins.
  • The probe enabled permanent staining of NO-modified proteins, compatible with standard immunostaining protocols.
  • Multiplex imaging demonstrated the involvement of NO in hypoglycemia-induced neurological injury, with evidence of NO-induced apoptosis and α-tubulin nitration.

Conclusions:

  • NOP-1 serves as a valuable tool for visualizing NO signaling and its interactions with proteins in biological systems.
  • The study provides direct evidence for NO's role in hypoglycemia-induced apoptosis, potentially through α-tubulin nitration.
  • This probe advances the field of NO imaging, offering new possibilities for studying NO-related biological processes.