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  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Prognostic Biomarker Nrg2 Correlates With Autophagy And Epithelial‑mesenchymal Transition In Breast Cancer

Prognostic biomarker NRG2 correlates with autophagy and epithelial‑mesenchymal transition in breast cancer

Ruijie Zhou1, Jinjin Dai1, Runlong Zhou1

  • 1Institute of Biology and Medicine, College of Life Science and Health, Wuhan University of Science and Technology, Wuhan, Hubei 430081, P.R. China.

Oncology Letters
|May 3, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Neuroregulatory protein 2 (NRG2) is downregulated in breast cancer (BRCA), acting as a tumor suppressor. Low NRG2 levels correlate with poor survival and promote cancer progression by affecting autophagy and immune responses.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Breast cancer (BRCA) is a significant global health concern for women.
  • The role of autophagy-related gene neuroregulatory protein 2 (NRG2) in BRCA progression and its molecular mechanisms are not well understood.

Purpose of the Study:

  • To investigate the expression, diagnostic value, and functional role of NRG2 in breast cancer.
  • To elucidate the molecular mechanisms by which NRG2 influences BRCA progression, focusing on autophagy and epithelial-mesenchymal transition (EMT).

Main Methods:

  • Gene expression analysis in BRCA tissues compared to normal controls.
  • Receiver operating characteristic (ROC) curve analysis for diagnostic value.
  • Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and Gene Set Enrichment Analysis (GSEA).
Keywords:
autophagybiomarkerbreast cancerepithelial-mesenchymal transition

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  • In vitro experiments involving NRG2 knockdown in BRCA cells to assess proliferation, invasion, and migration.

    • Main Results:

    • NRG2 expression was significantly downregulated in BRCA tissues, correlating with poorer survival rates.
    • NRG2 demonstrated good diagnostic value for distinguishing BRCA from normal tissues (AUC=0.932).
    • NRG2 and its regulated genes were enriched in autophagy and immune-related pathways, with NRG2 positively correlated with immune cells and checkpoint genes.
    • NRG2 depletion promoted BRCA cell proliferation, invasion, and migration, increasing autophagy (LC3-II) and EMT (vimentin) markers while decreasing P62 and E-cadherin.

    Conclusions:

    • NRG2 functions as a tumor suppressor in breast cancer.
    • NRG2 inhibits immune escape and anti-tumor immunity by regulating autophagy and EMT.
    • NRG2 holds potential as a prognostic biomarker and therapeutic target for BRCA.
    neuroregulatory protein 2