Related Experiment Video

Updated: Jun 27, 2025

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
09:40

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

12.2K

Complete genome sequence of the Microbacterium enclense bacteriophage phiMiGM15

Erika Camacho-Beltrán1,2, Juan José Morales-Aguilar3, Melina López-Meyer2

  • 1Laboratorio de Fitopatología, Unidad de Biotecnología Vegetal, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Zapopan, Jalisco, Mexico.

Microbiology Resource Announcements
|May 3, 2024
PubMed

Insights

We sequenced the phiMiGM15 bacteriophage, which infects Microbacterium enclense. This lytic siphovirus has a 48.6 kb genome with 81 predicted genes, showing similarity to other Microbacterium phages.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Bacteriophages are viruses that infect bacteria and play crucial roles in microbial ecosystems.
  • Lytic bacteriophages offer potential as therapeutic agents against bacterial infections.
  • Characterizing novel bacteriophages is essential for understanding phage diversity and host interactions.

Purpose of the Study:

  • To determine the complete genome sequence of phiMiGM15, a lytic bacteriophage.
  • To analyze the genomic features and potential functions of genes within the phiMiGM15 genome.
  • To compare phiMiGM15 with related bacteriophages for evolutionary insights.

Main Methods:

  • Isolation and purification of bacteriophage phiMiGM15 from its host.
  • Whole-genome sequencing using next-generation sequencing technologies.
  • Bioinformatic analysis for gene prediction and comparative genomics.

Main Results:

  • The complete genome sequence of phiMiGM15 was determined to be 48.6 kb in length.
  • Eighty-one putative genes were identified within the phiMiGM15 genome.
  • phiMiGM15 shares 28% genome coverage and 82.26% nucleotide identity with Microbacterium phage Caron (accession OQ190481.1).

Conclusions:

  • phiMiGM15 is a novel lytic bacteriophage infecting Microbacterium enclense, belonging to the siphovirus morphological group.
  • The genomic data provides a foundation for further functional studies of phiMiGM15.
  • Comparative analysis reveals evolutionary relationships between phiMiGM15 and other Microbacterium-infecting phages.
Keywords:
Microbacterium enclensebiological controldiseases of bean crops

More Related Videos

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.0K
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'08:31

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

10.9K

Related Concept Videos

Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.6K
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.8K