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A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
Published on: March 28, 2017
Dereplication Tools for Rhizophora mangle Extracts from Different Mangrove Areas and their Potential Against
Amanda Letícia da Silva Pontes1,2, Carla Monteiro Leal3, Manuela Pereira Lucas1
1Laboratório de Tecnologia em Produtos Naturais, Departamento de Farmácia, Faculdade de Ciências Biológicas e Saúde, Universidade do Estado do Rio de Janeiro (UERJ), Campus Zona Oeste, Campo Grande, Av. Manuel Caldeira de Alvarenga 1.203, Rio de Janeiro, RJ, 23070-200, Brazil.
Abstract:
Rhizophora extracts have several potential biological activities, and their metabolites can be used in the pharmaceutical industry. Extracts of Rhizophora species obtained from mangroves have shown prospective activity against Staphylococcus aureus. This study aimed to investigate the chemical profile of Rhizophora mangle leaves from fringe, basin, and transition mangrove zones and their bactericidal/bacteriostatic potential against S. aureus. R. mangle leaves were collected monthly in 2018 from litterfall in three different zones of the mangrove of Guaratiba State Reserve: fringe, basin, and transition. Extracts were prepared from the material collected in October and December for LC-HRMS/MS analysis, and dereplication was performed using a molecular library search and the classical molecular networking GNPS platform. The minimum inhibitory concentrations (MICs) of the aqueous extract of R. mangle against S. aureus were determined. No S. aureus growth was observed compared to the control for extracts collected from September to December. Different compounds were annotated in each region, yet a marked presence of phenolic compounds was noted, among them glycosylated flavonoid derivatives of quercetin and kaempferol. The results suggest bactericidal/bacteriostatic activity for extracts of R. mangle leaves collected in 2018 from three mangrove forest zones.
