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An In-Situ-Tag-Generation Proximity Labeling Technology for Recording Cellular Interactions

Yunyan Yao1, Ru Jia2, Chuanming Liu3,4

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 210023, Nanjing, P. R. China.

Angewandte Chemie (International Ed. in English)
|May 3, 2024
PubMed
Summary

We developed GalTag, a novel proximity labeling technology, to track cellular interactions in solid tissues. This method enables in situ tag generation, overcoming limitations of traditional methods and revealing Bacillus Calmette-Guérin invasion dynamics in mouse bladder tissue.

Keywords:
Bacillus Calmette-GuérinBio-orthogonal labelingCell interaction recordingEndogenous substrateGalactose oxidase

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