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Gold nanoparticle-decorated fluorine-doped tin oxide substrate for sensitive label-free OIRD microarray chips
Yuda Ren1, Meng Li1, Xiaoyi Li1
1Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, School of Materials and Energy, Southwest University, Chongqing, 400715, People's Republic of China.
Analytical and Bioanalytical Chemistry
|May 3, 2024
Summary
A new microarray chip using gold nanoparticle-decorated fluorine-doped tin oxide (AuNPs-FTO) enhances label-free detection. This biosensor achieves higher sensitivity and signal-to-noise ratio for detecting biomolecular interactions.
Area of Science:
- Biosensing
- Nanotechnology
- Surface science
Background:
- Oblique incidence reflectance difference (OIRD) is an emerging label-free detection technique for bio-affinity binding.
- The chip's interfacial architecture significantly impacts OIRD performance.
- Developing sensitive OIRD microarray chips is crucial for advancing real-time biological detection.
Purpose of the Study:
- To develop a highly sensitive label-free OIRD microarray chip.
- To investigate the role of gold nanoparticle decoration on the chip substrate.
- To enhance the signal-to-noise ratio and sensitivity of OIRD detection.
Main Methods:
- Fabrication of OIRD microarray chips using gold nanoparticle-decorated fluorine-doped tin oxide (AuNPs-FTO) slides.
- Characterization of chip performance using HRP-conjugated streptavidin as a model target.
- On-chip ELISA experiments and optical calculations to elucidate performance enhancements.
Main Results:
- The AuNPs-FTO chip exhibited a higher signal-to-noise ratio and improved sensitivity compared to FTO glass.
- A detection limit as low as 10 ng mL⁻¹ was achieved for the model target.
- Optical interference generated by the AuNP layer was identified as a key factor for enhanced OIRD sensitivity.
Conclusions:
- A sensitive label-free OIRD microarray chip was successfully developed using AuNPs-FTO substrates.
- The AuNP layer significantly enhances OIRD sensitivity through optical interference and increased probe density.
- This strategy offers a promising approach for developing advanced microarray chips for diverse applications.
