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Updated: Aug 2, 2026

Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
12:12

Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice

Published on: February 8, 2016

[Transformation of the yeast Hansenula polymorpha by a hybrid plasmid containing the fragment of host DNA]

E N Makhina, G F Nesterova, A V Grishin

    Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia
    |May 1, 1985
    PubMed

    Insights

    Hansenula polymorpha yeast can now be genetically modified using hybrid plasmid DNA. This breakthrough enables efficient DNA transformation for this yeast species.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Yeast Genetics

    Context:

    • Hansenula polymorpha is a methylotrophic yeast with industrial applications.
    • Genetic manipulation of H. polymorpha is crucial for strain improvement and metabolic engineering.
    • Previous transformation methods for H. polymorpha were limited.

    Purpose:

    • To develop an efficient DNA transformation method for Hansenula polymorpha.
    • To establish a stable gene transfer system for H. polymorpha using hybrid plasmids.
    • To investigate the maintenance and inheritance of hybrid plasmids in H. polymorpha transformants.

    Summary:

    • Spheroplasts of a Hansenula polymorpha strain lacking 2-isopropylmalate dehydrogenase were successfully transformed using a hybrid plasmid (pHRI).
    • This plasmid contains the LEU2 gene from Saccharomyces cerevisiae and a genomic DNA fragment from H. polymorpha.
    • High transformation frequencies (10^3 per microgram DNA) were achieved, with the plasmid maintained autonomously and stably inherited in a fraction of cells.

    Impact:

    • Hansenula polymorpha is now susceptible to hybrid plasmid-mediated gene transfer, expanding the toolkit for yeast genetic engineering.
    • This facilitates the development of novel H. polymorpha strains for biotechnological applications.
    • The established transformation protocol provides a foundation for future genetic studies and synthetic biology efforts in H. polymorpha.

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