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Updated: Jun 27, 2025

Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

Published on: January 20, 2023

Potential for targeting small heat shock protein modifications.

Binyou Wang¹, Matthew R Pratt¹

¹Department of Chemistry, University of Southern California, Los Angeles, CA 90089, USA.

Trends in Pharmacological Sciences

|May 4, 2024

Summary

Small heat shock proteins (sHSPs) are crucial for cellular stress responses and disease. Modulating their activity through small molecules targeting post-translational modifications offers a potential therapeutic strategy.

Keywords:

O-GlcNAc glycation heat shock proteins phosphorylation post-translational modifications protein aggregation

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Last Updated: Jun 27, 2025

Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

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Published on: January 20, 2023

Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay

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Published on: July 21, 2021

Intracellular Refolding Assay

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Published on: January 24, 2012

Area of Science:

Biochemistry
Molecular Biology
Cellular Biology

Background:

Small heat shock proteins (sHSPs) are vital molecular chaperones involved in cellular stress.
sHSPs play significant roles in the pathogenesis of various human diseases.
Post-translational modifications (PTMs) are known to affect sHSP function.

Purpose of the Study:

To explore the potential of small molecules in modulating sHSP activity.
To investigate the link between PTMs of sHSPs and their functional regulation.

Main Methods:

Characterization of direct effects of PTMs on specific sHSPs.
Exploration of small molecule-mediated modulation of sHSP-associated PTMs.

Main Results:

Established that PTMs directly impact the function of certain sHSPs.
Demonstrated the feasibility of indirectly regulating sHSP activity via small molecules targeting PTMs.

Conclusions:

sHSP activity can be modulated by targeting their post-translational modifications.
Small molecule-based strategies hold promise for therapeutic interventions in diseases involving sHSPs.