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Updated: Jun 27, 2025
07:14
The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
Published on: August 4, 2018
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De-nitrosylation Coordinates Appressorium Function for Infection of the Rice Blast Fungus
Hong Hu1, Wenhui He1, Zhiguang Qu1
1National Key Laboratory of Agricultural Microbiology and Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Summary
The rice blast fungus requires de-nitrosylation for appressorium formation. S-nitrosoglutathione reductase (GSNOR) regulates this process, highlighting potential fungal disease control strategies by disrupting nitric oxide (NO) homeostasis.
Area of Science:
- Molecular Biology
- Mycology
- Plant Pathology
Background:
- Nitric oxide (NO) is a crucial signaling molecule regulating development and stress responses.
- Protein S-nitrosylation, a key NO activity, has an unclear role in fungal pathogenesis.
- Appressorium formation in Magnaporthe oryzae is vital for rice infection.
Purpose of the Study:
- To investigate the role of de-nitrosylation in appressorium formation in Magnaporthe oryzae.
- To identify proteins regulated by S-nitrosylation and de-nitrosylation.
- To explore potential strategies for fungal disease control targeting NO homeostasis.
Main Methods:
- Utilized an indoTMT switch labeling proteomics technique.
- Analyzed S-nitrosylation sites in proteins crucial for appressorium formation.
- Investigated the impact of disrupting NO homeostasis (using NO donors, scavengers, and GSNOR inhibitors).
Main Results:
- De-nitrosylation is essential for functional appressorium formation in Magnaporthe oryzae.
- GSNOR-mediated de-nitrosylation mitigates nitrosative stress and promotes infection.
- Identified 741 S-nitrosylation sites on 483 proteins, including key appressorial proteins like Mgb1, MagB, Sps1, Cdc42, and septins.
- GSNOR-mediated removal of S-nitrosylation sites is critical for protein structure and appressorial function.
Conclusions:
- GSNOR-mediated de-nitrosylation is a key regulator of appressorium formation in Magnaporthe oryzae.
- Disrupting NO homeostasis through NO donors, scavengers, or GSNOR inhibitors offers promising avenues for fungal disease control.
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