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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 27, 2025

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Molecular cytometry for comprehensive immune profiling.

Pratip K Chattopadhyay¹

¹Talon Biomarkers, Whippany, NJ, United States.

Methods in Cell Biology

|May 5, 2024

Summary

This summary is machine-generated.

Molecular cytometry, a high-parameter single-cell analysis technology, measures proteins and transcripts simultaneously. This powerful immune monitoring tool offers deep insights into cellular function and disease.

Keywords:

Biomarkers Cytometry Immune monitoring Sequencing

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Last Updated: Jun 27, 2025

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

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Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR

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Published on: February 19, 2020

Area of Science:

Single-cell biology
Immunology
Molecular biology

Background:

High-parameter single-cell analysis is crucial for understanding complex biological systems.
Existing technologies have limitations in simultaneously measuring protein and transcript levels.
Immune monitoring requires detailed cellular characterization.

Purpose of the Study:

To describe the molecular cytometry technology platform.
To provide guidance on optimizing molecular cytometry experiments.
To discuss the applications and potential of molecular cytometry in research.

Main Methods:

Utilizes oligonucleotide-tagged antibodies for protein detection.
Combines protein measurement with targeted gene or whole transcriptome analysis.
Employs medium-throughput single-cell analysis (10-100K cells).

Main Results:

The platform enables simultaneous measurement of over 100 proteins and hundreds of genes or the whole transcriptome.
Demonstrates the power of molecular cytometry for immune monitoring.
Highlights applications and potential insights from molecular cytometry studies.

Conclusions:

Molecular cytometry (including CITE-Seq, REAP-Seq, AbSeq) is a powerful tool for single-cell analysis.
Optimization guidance and understanding of limitations are essential for effective use.
This technology offers significant potential for advancing immune monitoring and biological research.