Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Uncovering novel germline variants associated with testicular germ cell tumors by exome sequencing.

NAR cancer·2026

Same author

Protein-protein interaction detection in plants: from classical approaches to future perspectives.

Biologia futura·2026

Same author

Retrotransposon Expression Is Upregulated in Adulthood and Suppressed during Regeneration of the Limb in the Axolotl (Ambystoma mexicanum).

Advanced biology·2025

Same author

Multi-omic analyses reveal the unique properties of chia (Salvia hispanica) seed metabolism.

Communications biology·2023

Same author

Differential Expression of miRNAs Involved in Response to <i>Candidatus</i> Liberibacter asiaticus Infection in Mexican Lime at Early and Late Stages of Huanglongbing Disease.

Plants (Basel, Switzerland)·2023

Same author

Electronic and magnetic properties of TATA-DNA sequence driven by chemical functionalization.

Journal of computational chemistry·2023

Same journal

Brassinosteroid signalling suppresses chlorophyll biosynthesis via BGH2-GLK modules to ensure proper cotyledon greening during de-etiolation.

Plant & cell physiology·2026

Same journal

Conserved trans-regulatory logic and evolving cis-modules drive the diversification of inducible diterpenoid biosynthetic gene clusters across the Oryzoideae.

Plant & cell physiology·2026

Same journal

Vernalisation-induced changes to the Arabidopsis circadian clock require Polycomb Repressive Complex 2 and are FLC-independent.

Plant & cell physiology·2026

Same journal

PUB4 Activates Chitin Signaling by Ubiquitination-linked Degradation of The Immune Suppressor FERONIA.

Plant & cell physiology·2026

Same journal

Pyridinium Derivatives as Novel Fluorescent Probes for Xylem Staining in Plant Tissues.

Plant & cell physiology·2026

Same journal

Identification of a dominant genic male-sterility gene derived from the rice cultivar 'Lebed'.

Plant & cell physiology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 27, 2025

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Published on: March 16, 2010

Proposal of Reference Genes for Tomato Fruit Ripening qRT-PCR Data Normalization

Ricardo Rivera-Silva¹, Ricardo A Chávez Montes², Alejandra Cervera³

¹División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica (IPICYT), Camino a la Presa San José 2055, San Luis Potosí 78216, México.

Plant & Cell Physiology

|May 6, 2024

Summary

No abstract available in PubMed .

More Related Videos

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

Published on: November 30, 2015

Related Experiment Videos

Last Updated: Jun 27, 2025

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Published on: March 16, 2010

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

Published on: November 30, 2015