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Boronic Acid-Linked Cell-Penetrating Peptide for Protein Delivery.
1Chemistry Division, School of Advanced Sciences, Vellore Institute of Technology, Chennai Campus, Chennai, Tamilnadu 600127, India.
ACS Omega
|May 6, 2024
Summary
Researchers developed a novel cell-penetrating peptide (CPP) for enhanced protein delivery. This boronic acid-linked cyclic deca arginine (cR10) shows significantly improved delivery of ubiquitin into live cells compared to unmodified CPPs.
Area of Science:
- Biochemistry and Molecular Biology
- Cell Biology
- Biotechnology
Background:
- Functional protein delivery into live cells is crucial for research and therapeutics.
- Cell-penetrating peptides (CPPs) are established tools for delivering macromolecules like proteins into cells.
- CPPs have significant potential in protein therapeutics and understanding cellular mechanisms.
Purpose of the Study:
- To design and evaluate a novel cell-penetrating peptide (CPP) for efficient delivery of macromolecules, specifically proteins.
- To investigate the efficacy of a boronic acid-linked cyclic deca arginine (cR10) as a CPP for protein delivery.
- To compare the delivery efficiency of the modified CPP with its pristine counterpart.
Main Methods:
- Synthesis of boronic acid-linked cyclic deca arginine (cR10) and pristine cR10.
- Chemical synthesis of ubiquitin (Ub) for delivery studies.
- Live-cell imaging and quantification of ubiquitin delivery into U2OS cells using modified and unmodified cR10.
Main Results:
- Boronic acid-linked cR10 demonstrated significantly enhanced delivery of chemically synthesized ubiquitin into live U2OS cells.
- The modified cR10 exhibited a 3-fold higher delivery efficiency compared to pristine cR10.
- This highlights the improved functionality of the boronic acid modification for CPP-mediated protein delivery.
Conclusions:
- Boronic acid-linked cyclic deca arginine (cR10) is an effective CPP for enhanced functional protein delivery into live cells.
- The developed CPP offers a promising tool for advancing protein therapeutics and cellular mechanism studies.
- Future work includes developing an artificial intelligence/machine learning-based rationale for CPP design.
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