Related Experiment Video
Updated: Jun 27, 2025
12:05
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
Published on: October 1, 2017
8.1K
OneFlowTraX: a user-friendly software for super-resolution analysis of single-molecule dynamics and nanoscale
Leander Rohr1, Alexandra Ehinger1, Luiselotte Rausch1
1Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany.
Frontiers in Plant Science
|May 6, 2024
Summary
OneFlowTraX is a new open-source software that simplifies super-resolution microscopy data analysis. It automates single-molecule localization and tracking, making nanoscale cell biology research more accessible.
Area of Science:
- Cell Biology
- Microscopy
- Biophysics
Background:
- Super-resolution microscopy (SRM) offers nanoscale insights into cellular processes.
- Complex post-acquisition data analysis hinders widespread SRM adoption.
Purpose of the Study:
- Introduce OneFlowTraX, a user-friendly, open-source software for single-molecule localization microscopy (SMLM) data analysis.
- Automate and streamline single-molecule localization, tracking, and mobility/clustering analyses.
Main Methods:
- Developed OneFlowTraX with an intuitive graphical user interface.
- Implemented automated analysis for SMLM data, including sptPALM.
- Ensured data management adheres to FAIR principles.
Main Results:
- OneFlowTraX enables rapid analysis of millions of single molecules.
- Software facilitates extraction of diffusion and clustering parameters.
- Demonstrated utility with plant plasma membrane proteins (aquaporin, BRI1, RLP44) using various fluorophores.
Conclusions:
- OneFlowTraX democratizes SMLM data analysis through automation and ease of use.
- The software accelerates the understanding of molecular dynamics in living cells.
- Facilitates high-throughput, FAIR-compliant analysis of SMLM data.
Related Concept Videos
01:37Super-resolution Fluorescence Microscopy
7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
01:19Protein Dynamics in Living Cells
2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K