Related Experiment Video
Updated: Jun 27, 2025

Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
Leptin attenuates the osteogenic induction potential of BMP9 by increasing β-catenin malonylation modification via
Kai-Xin Ke1,2, Xiang Gao2,3, Lu Liu1,2
1Department of Pharmacology, School of Pharmacy, Chongqing Medical University, Chongqing 400016, People's Republic of China.
Abstract:
BMP9 has demonstrated significant osteogenic potential. In this study, we investigated the effect of Leptin on BMP9-induced osteogenic differentiation. Firstly, we found Leptin was decreased during BMP9-induced osteogenic differentiation and serum Leptin concentrations were increased in the ovariectomized (OVX) rats. Both in vitro and in vivo, exogenous expression of Leptin inhibited the process of osteogenic differentiation, whereas silencing Leptin enhanced. Exogenous Leptin could increase the malonylation of β-catenin. However, BMP9 could increase the level of Sirt5 and subsequently decrease the malonylation of β-catenin; the BMP9-induced osteogenic differentiation was inhibited by silencing Sirt5. These data suggested that Leptin can inhibit the BMP9-induced osteogenic differentiation, which may be mediated through reducing the activity of Wnt/β-catenin signalling via down-regulating Sirt5 to increase the malonylation level of β-catenin partly.
Related Concept Videos
Cell Specific Gene Expression
TGF - β Signaling Pathway
PI3K/mTOR/AKT Signaling Pathway
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathways
The JAK-STAT Signaling Pathway

