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Plasma-derived extracellular vesicles improve mice embryo development.

Maryam Javadi1,2, Mohammad Sadegh Gholami Farashah3,4, Leila Roshangar3,5

  • 1Department of Anatomical Sciences, Faculty of Medicine, Zanjan University of Medical Sciences (ZUMS), Zanjan, Iran. maryamjavadi22@yahoo.com.

Molecular Biology Reports
|May 6, 2024
PubMed
Summary

Plasma-derived extracellular vesicles (EVs) enhance mouse embryo development. Supplementing in vitro maturation media with these EVs improved fertilization and early embryo development rates significantly.

Keywords:
Electronic microscopyFertilizationOocyte maturationPlasma-derived EVs

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Area of Science:

  • Reproductive biology
  • Cell biology
  • Extracellular vesicles research

Background:

  • Investigating the impact of plasma-derived extracellular vesicles (EVs) on mammalian reproductive processes.
  • Comparing the efficacy of EVs versus conventional media in supporting early embryonic development.

Purpose of the Study:

  • To determine the effect of plasma-derived extracellular vesicles (EVs) on fertilization and early embryo development in mice.
  • To assess the potential of EVs as a supplement in assisted reproductive technologies.

Main Methods:

  • Isolation of extracellular vesicles (EVs) from mouse plasma via ultracentrifugation.
  • Characterization of EVs using dynamic light scattering (DLS) and transmission electron microscopy (TEM).
  • Western blotting to identify EV protein markers (e.g., CD82, HSP90) and assess their impact on reproductive proteins.

Main Results:

  • Plasma-derived EVs demonstrated positive effects on the fertilization rate in mice (P < 0.05).
  • Supplementation with EVs significantly increased early embryo development rates, including 8-cell, morula, and blastocyst stages (P < 0.01).
  • EVs were successfully incorporated into the oocyte cytoplasm, as visualized with DiI-labeled EVs.

Conclusions:

  • Supplementing in vitro maturation media with plasma-derived EVs is beneficial for mouse embryo development.
  • EVs hold promise as a novel component for improving the success rates of in vitro fertilization and embryo culture.