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[Indirect immunoelectron microscopic method of localization of albumin in mouse hepatocytes]
The mouse liver was fixed by 12 fixator variants on the basis of glutaraldehyde, paraformaldehyde, picric acid and lysin, and the localization of albumin was studied by the indirect immunoperoxidase method. The comparison of the results shows that immersion in 8% paraformaldehyde is a preferable method of fixation. Albumin was detected on ribosomes and membranes of the nuclear envelope, the coarse endoplasmic reticulum and in the matrix of the hepatocytic cytoplasm. It is suggested that albumin synthetized is directly discharged into cytosol and then into the extracellular space.
The mouse liver was fixed by 12 fixator variants on the basis of glutaraldehyde, paraformaldehyde, picric acid and lysin, and the localization of albumin was studied by the indirect immunoperoxidase method. The comparison of the results shows that immersion in 8% paraformaldehyde is a preferable method of fixation. Albumin was detected on ribosomes and membranes of the nuclear envelope, the coarse endoplasmic reticulum and in the matrix of the hepatocytic cytoplasm. It is suggested that albumin synthetized is directly discharged into cytosol and then into the extracellular space.