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Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

709
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
709

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Deciphering the TET3 interactome in primary thymic developing T cells.

Dimitris Theofilatos1, Tricia Ho2, Greg Waitt2

  • 1Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Iscience
|May 7, 2024
PubMed
Summary
This summary is machine-generated.

Ten-eleven translocation 3 (TET3) protein interacts with numerous partners in developing T cells. These interactions reveal TET3

Keywords:
BiochemistryMolecular biologyMolecular interactionProteomics

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Ten-eleven translocation (TET) proteins are DNA dioxygenases crucial for active DNA demethylation.
  • TET3 is highly expressed in developing T cells and implicated in T cell lineage specification.
  • TET-deficient mice show complex phenotypes, suggesting multifaceted roles for TET3 beyond demethylation.

Purpose of the Study:

  • To identify endogenous TET3 interacting partners in primary developing T cells using in vivo conditions.
  • To elucidate the broader biological functions of TET3 through its protein interactome.

Main Methods:

  • Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was employed.
  • Analysis was performed on primary developing T cells to capture endogenous interactions.

Main Results:

  • A comprehensive list of TET3 interacting partners was identified.
  • TET3 was found to participate in diverse cellular processes including transcriptional regulation, RNA polymerase elongation, splicing, DNA repair, and DNA replication.

Conclusions:

  • TET3 engages in a wide array of fundamental biological processes, extending beyond its known role in DNA demethylation.
  • The identified interactome provides a foundation for future research into TET3's precise mechanisms in T cell biology.