Sample multiplexing for retinal single-cell RNA-sequencing.

Area of Science:

• Genomics
• Molecular Biology
• Neuroscience

Background:

• Characterizing rare cell populations with microfluidic single-cell RNA sequencing (scRNA-seq) is challenging.
• Current enrichment and pooling methods obscure sample origin and phenotypic data, especially in high-variation experiments.

Conclusions:

• Sample multiplexing is a valuable method for increasing the precision of scRNA-seq analysis.
• This technique is particularly useful for studies involving rare cell populations, disease models, and human samples.