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Updated: Jun 27, 2025

Identification of Novel Regulators of Plant Transpiration by Large-Scale Thermal Imaging Screening in Helianthus Annuus

Identification of Novel Regulators of Plant Transpiration by Large-Scale Thermal Imaging Screening in Helianthus Annuus

Published on: January 30, 2020

Screening methods for thermotolerance in pollen.

Madeleine Stokes¹, Anja Geitmann¹

¹Department of Plant Science, Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Canada.

Annals of Botany

|May 7, 2024

Summary

This summary is machine-generated.

Plant reproduction struggles with heat stress, especially male gametophyte development. This review clarifies pollen viability and vigour assessments to aid crop breeding for climate change adaptation.

Keywords:

Fertilization heat stress plant reproduction pollen pollen development pollen morphology pollen tube pollen viability pollen vigour thermosensitivity thermotolerance

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Area of Science:

Plant reproductive biology
Environmental stress physiology
Climate change adaptation

Background:

Plant reproduction is sensitive to temperature stress, impacting crop yields and natural populations.
Elevated temperatures critically affect male gametophyte development, a key reproductive stage.
Existing studies lack consistent terminology for assessing pollen performance under stress.

Purpose of the Study:

To define and clarify the terms 'pollen viability' and 'pollen vigour'.
To review and summarize methodologies for assessing pollen performance under temperature stress.
To provide a foundation for consistent terminology in plant reproductive stress research.

Main Methods:

Review of scientific literature on plant reproduction and temperature stress.
Analysis of methodologies used to assess pollen viability (cellular features, germination assays).
Analysis of methodologies used to assess pollen vigour (in vitro growth, in vivo pollen tube tracking).

Main Results:

Pollen viability is often inferred from cellular features, while germination assays offer direct measurement.
Pollen vigour assessment includes pollen tube growth rate and morphological/growth pattern analysis.
Inconsistent application of 'viability' and 'vigour' terms complicates comparative studies.

Conclusions:

Standardized definitions of pollen viability and vigour are crucial for accurate research.
Consistent application of assessment methodologies will improve understanding of plant reproductive stress responses.
This work supports the development of climate-resilient crops and prediction of natural population dynamics.