Micelle-based fluorogenic sensing of trypsin: a sensitive method in pancreatic disease diagnosis

Protamine-mediated micellar aggregates, featuring an AIE-based fluorescent sensor, facilitate efficient detection of trypsin activity. This method enables the detection of trypsin at exceptionally low concentrations (0.01-0.1 μg mL^-1) in urine, demonstrating its potential for early clinical diagnosis of trypsin-related pancreatic diseases.