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Simultaneous Dopamine and Serotonin Monitoring in Freely Moving Crayfish Using a Wireless Electrochemical Sensing
Jinjing Han1,2,3, Ta-Wen Ho4,5, Justin M Stine1,2,3
1Department of Electrical and Computer Engineering, University of Maryland, College Park, Maryland 20742, United States.
ACS Sensors
|May 7, 2024
Summary
Researchers developed a novel bioelectronic device for real-time, simultaneous monitoring of dopamine (DA) and serotonin (5-HT) in freely moving animals. This tool enhances understanding of neurotransmitter dynamics and their impact on behavior.
Area of Science:
- Neuroscience
- Bioelectronics
- Analytical Chemistry
Background:
- Dopamine (DA) and serotonin (5-HT) are crucial neurotransmitters regulating physiological and behavioral processes.
- Simultaneous real-time monitoring of DA and 5-HT dynamics in freely moving animals is essential for understanding behavior but is limited by current bioelectronic tools.
- Existing sensor sensitivity and miniaturization challenges hinder in vivo simultaneous neurotransmitter detection.
Purpose of the Study:
- To develop a highly sensitive and selective semi-implantable electrochemical device for simultaneous in vivo monitoring of dopamine and serotonin.
- To create a miniaturized, untethered system minimizing impact on animal behavior.
- To validate the device's capability in detecting DA and 5-HT dynamics in freely behaving animals.
Main Methods:
- Integration of a multi-surface-modified carbon fiber microelectrode with a miniaturized potentiostat module.
- Electrochemical modification of microelectrodes to enhance sensitivity, selectivity, and antifouling properties.
- Development of a lightweight, custom potentiostat for untethered measurements in crayfish hemolymph.
Main Results:
- The developed microsystem achieved simultaneous detection of DA and 5-HT with high sensitivities (48.4 nA/μM for DA, 133.0 nA/μM for 5-HT) within submicromolar ranges.
- The semi-implantable biosensor system demonstrated significant increases in oxidation peak currents upon DA and 5-HT injections.
- The device successfully monitored neurotransmitter dynamics in freely behaving crayfish underwater.
Conclusions:
- The novel semi-implantable electrochemical device enables sensitive and selective simultaneous in vivo monitoring of DA and 5-HT.
- This technology provides a valuable tool for studying the comodulation of DA and 5-HT in freely behaving animals.
- The system advances our understanding of the neurobiological mechanisms underlying behavior.
Keywords:
biosensingdopamineelectrochemistryfreely moving animalimplantablein vivoserotoninwireless system
