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Jacalin: an IgA-binding lectin.

M C Roque-Barreira, A Campos-Neto

    Journal of Immunology (Baltimore, Md. : 1950)
    |March 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Jackfruit lectin, jacalin, precipitates immunoglobulin A (IgA) from serum and secretions. This D-Gal binding lectin offers a new method for purifying IgA using affinity chromatography.

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    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • Artocarpus integrifolia (jackfruit) seeds contain a lectin, jacalin, known to activate T and B cells.
    • High concentrations of jacalin induce significant precipitation in cell cultures.
    • The nature of this precipitate was investigated in relation to serum proteins.

    Purpose of the Study:

    • To identify the serum component(s) precipitated by jacalin.
    • To explore the potential of jacalin for immunoglobulin purification.
    • To characterize jacalin's specificity for immunoglobulin A (IgA).

    Main Methods:

    • Jacalin was incubated with human serum and colostrum.
    • Precipitates were analyzed for immunoglobulin content (IgA, IgG, IgM).
    • Affinity chromatography was developed using jacalin for IgA purification.

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    Main Results:

    • Jacalin specifically precipitates IgA from human serum and colostrum.
    • No significant precipitation of IgG or IgM by jacalin was observed.
    • A simple and reliable affinity chromatography method for IgA purification was established.

    Conclusions:

    • Jacalin exhibits high specificity for IgA.
    • Jacalin is a valuable tool for the purification of serum and secretory IgA.
    • This D-Gal binding lectin facilitates studies on IgA structure and function.