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Related Experiment Video

Updated: Jun 27, 2025

Genetic Modification and Recombination of Salivary Gland Organ Cultures
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Salivary Gland Tissue Recombination Can Modify Cell Fate.

R Sekiguchi1, D Martin2, A D Doyle1,3

  • 1Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.

Journal of Dental Research
|May 8, 2024
PubMed
Summary
This summary is machine-generated.

Mesenchyme influences salivary gland development, with some epithelial cells showing plasticity and others committed fates. Tissue recombination revealed bidirectional induction between salivary gland epithelium and mesenchyme.

Keywords:
developmental biologyepithelial-mesenchymal interactiongene expressionmorphogenesissalivary physiologysingle cell RNAseq

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genomics

Background:

  • Mesenchyme is crucial for ectodermal organ induction.
  • The specific roles of mesenchyme in organ-specific epithelial fate determination are not fully understood.

Purpose of the Study:

  • To investigate the roles of tissue interactions in cellular differentiation during salivary gland development.
  • To characterize molecular changes in epithelial and mesenchymal cells during heterotypic tissue recombination.

Main Methods:

  • Ex vivo recombination of embryonic mouse parotid (serous) and submandibular (mucous) salivary gland tissues.
  • Single-cell RNA sequencing and imaging analyses.

Main Results:

  • Recombined salivary epithelium showed partial induction of gland-specific acinar and myoepithelial cell markers.
  • Parotid epithelium with submandibular mesenchyme expressed mucous acinar genes and induced myoepithelial cells.
  • Submandibular epithelium with parotid mesenchyme maintained mucous acinar and myoepithelial marker expression.
  • Evidence of bidirectional induction, with mesenchymal cells altering gene expression in response to epithelial signals.

Conclusions:

  • Some epithelial cells retain plasticity, allowing fate modification by mesenchymal signaling, while others are already committed.
  • Tissue recombination in salivary glands demonstrates reciprocal signaling and molecular plasticity between epithelial and mesenchymal components.