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Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure
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Effects of phantom microstructure on their optical properties.

Jošt Stergar1,2, Rok Hren2,3,4, Matija Milanič1,2

  • 1"Jožef Stefan" Institute, Ljubljana, Slovenia.

Journal of Biomedical Optics
|May 8, 2024
PubMed
Summary

Understanding tissue phantom microstructure is key for accurate biomedical optics. This study links phantom structure to optical properties, enabling precise material characterization without calibration for robust clinical applications.

Keywords:
hyperspectral imagingmicroscopyspectroscopytissue phantoms

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Area of Science:

  • Biomedical Optics
  • Materials Science

Background:

  • Developing stable, robust, and affordable tissue-mimicking phantoms is crucial for advancing biomedical optics applications.
  • A deep understanding of phantom structure and optical properties is essential for their successful implementation.

Purpose of the Study:

  • To characterize the structural and optical properties of PlatSil SiliGlass phantoms.
  • To investigate the impact of phantom microstructure on overall optical properties.

Main Methods:

  • Utilized scanning electron microscopy (SEM) for structural analysis.
  • Employed hyperspectral imaging (HSI) and spectroscopy for optical property measurements.
  • Combined Mie theory modeling and inverse Monte Carlo simulations for numerical analysis.

Main Results:

  • SEM revealed microsphere size variations and aggregation, impacting optical properties.
  • Spectroscopy quantified absorption coefficients, while size distribution determined scattering coefficients.
  • Inverse problem solutions accurately predicted component volume fractions and explained spectral features.
  • HSI microscopy confirmed single-scattering events consistent with phantom microstructure.

Conclusions:

  • Knowledge of phantom microstructure allows for absolute material constitution measurements without prior calibration.
  • A link exists between microstructural details and macroscopically observable optical properties.
  • Precise phantom component constitution aids in understanding overall optical behavior for biomedical applications.