Related Experiment Video

Updated: Jun 27, 2025

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
09:29

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Published on: March 20, 2020

17.6K

The functional role of Nudt2 in human triple negative breast cancer

Rasha Abu-Rahmah1,2, Hovav Nechushtan1, Sanaa Hidmi2

  • 1Department of Oncology, Hadassah Hebrew University Medical Center, Jerusalem, Israel.

Frontiers in Oncology
|May 8, 2024
PubMed

Insights

Nudix hydrolase 2 (Nudt2) promotes triple-negative breast cancer (TNBC) growth. Higher Nudt2 expression in tumors correlates with reduced proliferation, migration, invasion, and tumorigenicity in TNBC cell lines, indicating its oncogenic role.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Nudix hydrolase 2 (Nudt2) primarily hydrolyzes the secondary messenger diadenosine 5', 5'''-p1, p4-tetraphosphate (Ap4A).
  • The specific role of Nudt2 in breast cancer, particularly triple-negative breast cancer (TNBC), remains largely unexplored.

Purpose of the Study:

  • To investigate the expression levels of Nudt2 in human invasive ductal carcinoma tissues.
  • To elucidate the functional role of Nudt2 in the progression of human triple-negative breast cancer (TNBC) cell lines.

Main Methods:

  • Quantitative analysis of Nudt2 expression in invasive ductal carcinoma and normal breast tissues.
  • Functional assays in TNBC cell lines, including Nudt2 knockdown, proliferation assays (Ki67), cell cycle analysis, migration and invasion assays, and anchorage-independent growth assessments.

Main Results:

  • Significantly elevated Nudt2 expression was detected in invasive ductal carcinoma tissues compared to normal breast tissues.
  • Nudt2 knockdown in TNBC cell lines led to reduced cellular proliferation (Ki67 marker), G0/G1 phase cell cycle arrest, decreased migration and invasion, and impaired tumorigenicity and anchorage-independent growth.

Conclusions:

  • Nudt2 is significantly upregulated in human invasive ductal carcinoma.
  • Nudt2 plays a crucial role in promoting TNBC cell proliferation, migration, invasion, and tumorigenicity, highlighting its potential as a therapeutic target.
Keywords:
Anchorage-independent growthNUDT2invasionmigrationtriple negative breast cancer (TNBC)

More Related Videos

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

2.6K
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

8.0K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.8K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.8K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
7.4K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.6K