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Related Experiment Video

Updated: Jun 26, 2025

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

Neovascularization by DPSC-ECs in a Tube Model for Pulp Regeneration Study.

Y Zhang¹, J Liu¹, I J de Souza Araujo¹

¹Departments of Bioscience Research and Endodontics, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, USA.

Journal of Dental Research

|May 8, 2024

Summary

This summary is machine-generated.

Researchers developed a novel tube model to study vascularization in dental pulp regeneration. Human dental pulp stem cell-derived endothelial-like cells formed vessel-like structures in vitro, showing promise for regenerative dentistry.

Keywords:

dental pulp endothelial cells fibrin hydrogels in vitro regeneration

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Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

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Last Updated: Jun 26, 2025

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

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Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model

Published on: September 9, 2011

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

Area of Science:

Biomaterials science
Regenerative medicine
Endothelial cell biology

Background:

Studying neovascularization in cell-based pulp regeneration is challenging.
A simplified in vitro model is needed for direct visualization of vascularization.

Purpose of the Study:

To develop and validate a tube model simulating root canal space for studying vascularization in vitro.
To assess the vascularization potential of endothelial-like cells derived from human dental pulp stem cells (DPSC-ECs).

Main Methods:

DPSC-ECs were generated and characterized using endothelial cell markers.
Cells were cultured in fibrin gel within a custom-designed tube model mimicking root canal space.
Vascular network formation was assessed via microscopy and histological analysis.

Main Results:

DPSC-ECs formed tubular structures resembling blood vessels in the 3D fibrin gel system.
Vessel-like networks were observed within 1-3 days of culture.
Histological analysis confirmed the presence of pulp-like tissue with vascular structures within the tube model.

Conclusions:

The developed tube model effectively simulates root canal space for in vitro vascularization studies.
DPSC-ECs demonstrate the capacity to form vascular structures and survive in a 3D fibrin gel environment.
This model provides a valuable tool for advancing research in pulp regeneration and vascular formation.