Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic Performance of Parathyroid CT and CT-Guided Targeted Parathyroidectomy in MEN1-Associated Primary Hyperparathyroidism.

Annales d'endocrinologie·2026
Same author

Concordance of conventional two-tier HER2 classification versus three-tier classification (including HER2-low status) in invasive breast carcinoma: a retrospective real-world analysis.

Journal of clinical pathology·2026
Same author

H3K27M-altered diffuse midline glioma: Experience and outcomes from a low-middle income country setting in the contemporary era.

Neuro-oncology advances·2026
Same author

Establishment and Molecular Characterization of a Short-Term Primary Culture Derived From Invasive Micropapillary Carcinoma of the Breast.

Cell biology international·2026
Same author

Two rare cases of FET::CREB epithelioid mesenchymal tumors, displaying atypical immunophenotype (myoepithelial-like), unraveled on next-generation sequencing.

Virchows Archiv : an international journal of pathology·2026
Same author

Small Cell Transformation of Metachronous ALK + ve Lung and Prostatic Adenocarcinoma following Initial Response to Ceritinib and Androgen Deprivation Therapies and Metastases to Two Different Organs: Guidance through FDG-PET/CT and <sup>68</sup> Ga-PSMA-11 PET-CT and Biopsy-Immunohistochemistry Correlation.

World journal of nuclear medicine·2026

Related Experiment Video

Updated: Jun 26, 2025

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

8.6K

Microfluidics-based EGFR mutation detection and its implication in the resource-limited clinical setting.

Pradnya Joshi1, Prachi Gogte1, Trupti Pai2

  • 1Molecular Pathology Division, Department of Pathology, Tata Memorial Hospital, Homi Bhabha National Institute, Mumbai, India.

International Journal of Experimental Pathology
|May 8, 2024
PubMed
Summary

A new microfluidics platform accurately detects epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) patients. This automated system offers a rapid, reliable diagnostic tool, especially beneficial in resource-limited settings for guiding targeted therapy.

Keywords:
EGFR mutation detectionNGSmicrofluidics technologymolecular diagnosisscreening of NSCLC patientstumor contentvariant allele frequency

More Related Videos

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
11:11

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

16.8K
A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction
10:35

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

Published on: April 5, 2018

10.3K

Related Experiment Videos

Last Updated: Jun 26, 2025

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

8.6K
Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
11:11

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

16.8K
A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction
10:35

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

Published on: April 5, 2018

10.3K

Area of Science:

  • Oncology
  • Molecular Diagnostics
  • Biotechnology

Background:

  • Management of lung cancer necessitates epidermal growth factor receptor (EGFR) mutational analysis for tyrosine kinase inhibitor (TKI) therapy selection.
  • Accurate and efficient EGFR mutation detection is crucial for prognostic stratification and personalized treatment in non-small cell lung cancer (NSCLC).

Purpose of the Study:

  • To evaluate the performance and clinical significance of an automated microfluidics-based platform for EGFR mutation detection.
  • To assess the platform's concordance with conventional methods and its utility in routine diagnostics.

Main Methods:

  • A two-phase study involving 174 formalin-fixed, paraffin-embedded (FFPE) NSCLC samples.
  • Phase I: Validation against real-time PCR and next-generation sequencing (NGS).
  • Phase II: Application of the microfluidics platform in routine diagnostics.

Main Results:

  • The microfluidics platform showed 96.5% concordance with real-time PCR and 89.2% with NGS.
  • It demonstrated 88.23% sensitivity and 100% specificity for EGFR mutations.
  • The platform generated valid results in 94% of samples in Phase II, detecting mutations in 41% and requiring minimal tissue (2 sections, 5μm thickness).

Conclusions:

  • The automated microfluidics platform is a rapid, walkaway solution for EGFR mutation detection with minimal technical skill and tissue requirements.
  • It is a reliable diagnostic platform, particularly suitable for resource-limited settings, enabling efficient detection of actionable mutations.
  • The study facilitated an algorithm for effective NSCLC screening, aiding judicious case triaging before multigene testing.