Among- and Within-Individual Variance in Metabolic Thermal Reaction Norms

AbstractIn ectotherms, temperature has a strong effect on metabolic rate (MR), yet the extent to which the thermal sensitivity of MR varies among versus within individuals is largely unknown. This is of interest because significant among-individual variation is a prerequisite for the evolution of metabolic thermal sensitivity. Here, we estimated the repeatability (R) of the thermal sensitivity of MR in individual virgin, adult male Drosophila melanogaster ($N=316$) by taking repeated overnight measures of their MRs at two temperatures (~24°C and ~27°C). At the population level, thermal sensitivity decreased with locomotor activity, and older individuals showed a higher thermal sensitivity of MR than younger individuals. Taking these effects (and body mass) into account, we detected significant repeatability in both the centered intercept ($R_{\mathrm{int}}=0.52\pm 0.04$) and the slope ($R_{\mathrm{slp}}=0.21\pm 0.07$) of the metabolic thermal reaction norms, which respectively represent average MR and thermal sensitivity of MR. Furthermore, individuals with a higher overall MR also displayed greater increases in MR as temperature increased from ~24°C to ~27°C ($r_{\mathrm{ind}}=0.32\pm 0.14$). Average MR and thermal sensitivity of MR were also positively correlated within individuals ($r_e=0.15\pm 0.07$). Our study represents a point of departure for future larger studies, in which more complex protocols (e.g., wider temperature range, breeding design) can be applied to quantify the causal components of variation in thermal sensitivity that are needed to make accurate predictions of adaptive responses to global warming.