Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

1.4K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K
Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

223
Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
223
pH Regulation in Cells01:28

pH Regulation in Cells

6.0K
pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
6.0K
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

177
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
177

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combined lactate- and phosphate-dependent cytoplasmic acidification drives <i>Mycobacterium tuberculosis</i> growth arrest at acidic pH.

bioRxiv : the preprint server for biology·2026
Same author

Defining the Mechanism of Action and Resistance of New <i>Mycobacterium abscessus</i> MmpL3 Inhibitors.

ACS chemical biology·2026
Same author

<i>Mycobacterium tuberculosis</i> growth arrest on propionate at acidic pH is suppressed by mutations in <i>phoPR</i> and pyrazinamide treatment.

mBio·2025
Same author

<i>Mycobacterium tuberculosis</i> growth arrest on propionate at acidic pH is suppressed by mutations in <i>phoPR</i> and pyrazinamide treatment.

bioRxiv : the preprint server for biology·2025
Same author

Defining the mechanism of action of the nitrofuranyl piperazine HC2210 against Mycobacterium abscessus.

npj antimicrobials and resistance·2025
Same author

Genetic and Cheminformatic Characterization of <i>Mycobacterium tuberculosis</i> Inhibitors Discovered in the Molecular Libraries Small Molecule Repository.

ACS infectious diseases·2025

Related Experiment Video

Updated: Jun 26, 2025

Growth of Mycobacterium tuberculosis Biofilms
09:03

Growth of Mycobacterium tuberculosis Biofilms

Published on: February 15, 2012

23.8K

Targeting Mycobacterium tuberculosis pH-driven adaptation.

Shelby J Dechow1, Robert B Abramovitch1

  • 1Department of Microbiology, Genetics and Immunology, Michigan State University, East Lansing, MI 48824, USA.

Microbiology (Reading, England)
|May 8, 2024
PubMed
Summary

Mycobacterium tuberculosis adapts to acidic environments within macrophages, impacting its growth and antibiotic tolerance. Targeting these pH-driven adaptations offers potential new therapeutic strategies against tuberculosis.

Keywords:
Mycobacterium tuberculosisenvironmental cuespathogenesistherapeutics

More Related Videos

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

7.8K
Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution
08:11

Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution

Published on: June 14, 2024

739

Related Experiment Videos

Last Updated: Jun 26, 2025

Growth of Mycobacterium tuberculosis Biofilms
09:03

Growth of Mycobacterium tuberculosis Biofilms

Published on: February 15, 2012

23.8K
A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

7.8K
Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution
08:11

Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution

Published on: June 14, 2024

739

Area of Science:

  • Microbiology
  • Pathogenesis
  • Molecular Biology

Background:

  • Mycobacterium tuberculosis (Mtb) encounters acidic environments in host macrophages, a key factor in its pathogenesis.
  • Acidic pH triggers significant transcriptional and metabolic changes in Mtb, influencing its survival and virulence.

Purpose of the Study:

  • To review the mechanisms Mtb uses to regulate growth and metabolism under acidic conditions.
  • To explore the therapeutic potential of targeting Mtb's pH-dependent adaptation pathways.

Main Methods:

  • Literature review of studies on Mtb's response to acidic pH.
  • Analysis of transcriptional and metabolic remodeling in Mtb at low pH.
  • Examination of virulence studies involving pH-adaptation mutants.

Main Results:

  • Acidic pH adaptation in Mtb leads to slower growth, increased pathogenesis, and enhanced antibiotic tolerance.
  • Mtb mutants lacking pH-dependent adaptations show reduced virulence in host models.
  • Compounds targeting pH-driven pathways or exhibiting pH-dependent activity are emerging as potential therapeutics.

Conclusions:

  • Mtb's adaptation to acidic host niches is crucial for its survival and virulence.
  • Disrupting these pH-dependent mechanisms presents a promising avenue for novel anti-tuberculosis therapies.