Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Col8a1 Is A Potential Prognostic Biomarker Associated With Migration, Proliferation, And Tumor Microenvironment In Glioma

COL8A1 is a potential prognostic biomarker associated with migration, proliferation, and tumor microenvironment in glioma

Mingkai Liu1, Weiye Liang2, Yuling Su3

  • 1Department of Neurobiology, School of Medicine, South China University of Technology, Guangzhou, China.

Experimental Cell Research
|May 8, 2024

Related Experiment Videos

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
06:32

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Published on: January 9, 2019

7.9K
Translational Orthotopic Models of Glioblastoma Multiforme
07:37

Translational Orthotopic Models of Glioblastoma Multiforme

Published on: February 17, 2023

2.7K
Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies
07:47

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

1.5K

View abstract on PubMed

Summary
This summary is machine-generated.

Collagen type VIII alpha 1 chain (COL8A1) promotes glioblastoma growth and invasion by inhibiting apoptosis and altering the tumor immune microenvironment. COL8A1 may serve as a prognostic marker and therapeutic target for glioblastoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Glioblastoma (GBM) is an aggressive brain tumor with poor prognosis and unknown molecular drivers.
  • Identifying novel prognostic markers and therapeutic targets is critical for improving GBM patient outcomes.

Purpose of the Study:

  • To identify key genes involved in glioblastoma progression using bioinformatics and experimental approaches.
  • To investigate the functional role and molecular mechanisms of COL8A1 in glioblastoma.
  • To analyze the association between COL8A1 and the tumor immune microenvironment (TME) in glioma.

Main Methods:

  • Weighted gene co-expression network analysis (WGCNA), Differentially Expressed Genes (DEGs), and LASSO-COX regression were used to identify candidate genes.
  • Functional experiments (in vitro and in vivo), RNA sequencing, and immunohistochemistry were performed to study COL8A1.
Keywords:
COL8A1ERKEpithelial-mesenchymal transitionGlioblastoma

Related Experiment Videos

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
06:32

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Published on: January 9, 2019

7.9K
Translational Orthotopic Models of Glioblastoma Multiforme
07:37

Translational Orthotopic Models of Glioblastoma Multiforme

Published on: February 17, 2023

2.7K
Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies
07:47

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

1.5K
  • Immune infiltration analysis was conducted to assess the correlation between COL8A1 and immune cells.

    • Main Results:

    • COL8A1 was identified as a significant risk gene for glioblastoma.
    • COL8A1 expression inhibited apoptosis and promoted glioblastoma cell migration, invasion, and proliferation.
    • COL8A1 was associated with glioma grade, prognosis, and TME, promoting M2 macrophage polarization and suppressing M1-like macrophages.

    Conclusions:

    • COL8A1 plays a crucial role in glioblastoma progression, potentially through epithelial-mesenchymal transition and ERK pathway activation.
    • COL8A1 influences the tumor immune microenvironment by modulating macrophage polarization.
    • COL8A1 represents a potential prognostic biomarker and therapeutic target for glioblastoma.
    SHC1
    Tumor immune microenvironment