Related Experiment Video
Updated: Jun 26, 2025
08:25
Development of Heterogeneous Enantioselective Catalysts using Chiral Metal-Organic Frameworks MOFs
Published on: January 17, 2020
7.3K
Enhancing Substrate Channeling with Multi-Enzyme Architectures in Hydrogen-Bonded Organic Frameworks
Vahide Oskoei1, Motilal Mathesh1, Wenrong Yang1
1Centre for Sustainable Products School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, 3216, Australia.
Chemistry (Weinheim an Der Bergstrasse, Germany)
|May 8, 2024
Summary
Hydrogen-bonded organic frameworks (HOF) offer a stable, metal-free platform for enzyme stabilization. These porous structures enhance enzyme activity and cascade reactions under extreme conditions.
Area of Science:
- Materials Science
- Biotechnology
- Chemical Engineering
Background:
- Hydrogen-bonded organic frameworks (HOF) are a novel class of crystalline, metal-free porous materials.
- HOFs exhibit unique needle-like structures beneficial for enzyme stabilization and molecular transfer in cascade reactions.
Purpose of the Study:
- To synthesize and evaluate the stability of HOFs under extreme conditions (pH and temperature).
- To investigate the one-pot encapsulation of single and bi-enzyme systems within HOFs.
- To assess the performance of encapsulated enzymes in cascade reactions.
Main Methods:
- Systematic synthesis of HOFs.
- Stability testing of HOFs across a wide pH range (1-14) and elevated temperatures (up to 115°C).
- One-pot encapsulation of glucose oxidase (GOX) and bi-enzyme systems.
- Activity assays for encapsulated enzymes and cascade reactions.
Main Results:
- HOFs demonstrated remarkable stability across pH 1-14 and temperatures up to 115°C.
- Encapsulated glucose oxidase retained 80-90% activity at 70°C and pH 11.
- HOF-encapsulated bi-enzyme systems exhibited near-zero transient time and a 4.25-fold increase in catalytic activity due to enhanced substrate channelling.
Conclusions:
- HOFs provide a robust and versatile platform for enzyme encapsulation and stabilization under harsh conditions.
- The facile synthesis and demonstrated stability of HOFs open avenues for advanced biocatalytic applications.
- Enhanced substrate channelling in HOF-encapsulated cascade reactions significantly boosts catalytic efficiency.
More Related Videos
Related Concept Videos
01:13Introduction to Mechanisms of Enzyme Catalysis
8.1K
For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
8.1K
02:34Enzymes
81.5K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
81.5K
02:34Regioselectivity and Stereochemistry of Acid-Catalyzed Hydration
8.4K
The rate of acid-catalyzed hydration of alkenes depends on the alkene's structure, as the presence of alkyl substituents at the double bond can significantly influence the rate.
8.4K
02:17Reduction of Alkenes: Asymmetric Catalytic Hydrogenation
3.3K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.3K
02:13Reduction of Alkenes: Catalytic Hydrogenation
12.0K
Alkenes undergo reduction by the addition of molecular hydrogen to give alkanes. Because the process generally occurs in the presence of a transition-metal catalyst, the reaction is called catalytic hydrogenation.
Metals like palladium, platinum, and nickel are commonly used in their solid forms — fine powder on an inert surface. As these catalysts remain insoluble in the reaction mixture, they are referred to as heterogeneous catalysts.
The hydrogenation process takes place on the...
Metals like palladium, platinum, and nickel are commonly used in their solid forms — fine powder on an inert surface. As these catalysts remain insoluble in the reaction mixture, they are referred to as heterogeneous catalysts.
The hydrogenation process takes place on the...
12.0K
02:45Acid-Catalyzed Hydration of Alkenes
14.0K
Alkenes react with water in the presence of an acid to form an alcohol. In the absence of acid, hydration of alkenes does not occur at a significant rate, and the acid is not consumed in the reaction. Therefore, alkene hydration is an acid-catalyzed reaction.
14.0K