Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

760
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
760
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

539
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
539
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

1.2K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a low-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.
To...
1.2K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.3K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lipid bilayers determine allostery but not intrinsic affinity of cAMP to pacemaker channels.

Nature communications·2026
Same author

Label-Free Optical Differentiation of Single Diffusing Amino Acids at Picomolar Concentrations.

Research square·2026
Same author

Molecule by Molecule Characterization of a Polymer Molecular Mass Distribution via Mass Photometry.

Angewandte Chemie (International ed. in English)·2026
Same author

A Fluorogenic Reaction for Monitoring Cross-Coupling with Turn-On Ratio Greater than a Hundred Thousand.

Chemical & biomedical imaging·2026
Same author

Single-Particle Photothermal Microscopy Using On-Chip Silicon Nitride Microring Resonators.

The journal of physical chemistry. A·2025
Same author

Alternative splicing of synaptotagmin 7 regulates oligomerization and short-term synaptic plasticity.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jun 26, 2025

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
09:04

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Published on: April 18, 2019

12.4K

Label-free detection and profiling of individual solution-phase molecules.

Lisa-Maria Needham1,2,3, Carlos Saavedra1, Julia K Rasch1

  • 1Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.

Nature
|May 8, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed a new label-free single-molecule detection method. This technique uses microcavities to analyze biomolecule conformation and dynamics in solution with high sensitivity.

More Related Videos

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
08:06

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

9.2K
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

35.2K

Related Experiment Videos

Last Updated: Jun 26, 2025

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
09:04

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Published on: April 18, 2019

12.4K
Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
08:06

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

9.2K
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

35.2K

Area of Science:

  • Biophysics
  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Chemistry and biology in solution rely on conformational dynamics and complexation.
  • Single-molecule techniques are vital for resolving molecular diversity.
  • Label-free methods enhance single-molecule measurement capabilities.

Purpose of the Study:

  • Develop a label-free single-molecule method to reveal molecular conformation in solution.
  • Provide a new microscopic perspective with unprecedented detail.
  • Enable detailed analysis of biomolecular behavior and function.

Main Methods:

  • Utilized enhanced light-molecule interactions in high-finesse fiber-based Fabry-Pérot microcavities.
  • Employed a new molecular velocity filter window and dynamic thermal priming mechanism.
  • Leveraged Pound-Drever-Hall (PDH) cavity locking to suppress environmental noise.

Main Results:

  • Detected individual, unlabelled biomolecules as small as 1.2 kDa (ten-amino-acid peptide) with signal-to-noise ratios >100.
  • Obtained 2D intensity and temporal profiles, distinguishing subpopulations in mixed samples.
  • Observed a linear relationship between passage time and molecular radius, enabling conformation and diffusion analysis.
  • Resolved mixtures of biomolecule isomers with identical weight and composition but different conformations.

Conclusions:

  • The developed method offers a powerful new tool for analyzing molecular conformation, diversity, and dynamics in solution.
  • This technique provides crucial information about diffusion and solution-phase conformation.
  • Potential broad applications exist in the life and chemical sciences for in vitro analysis.