Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Suppression of ATM kinase signaling accelerates cellular senescence.

Stem cell reports·2026
Same author

Localization of Puncta Adherentia Junctions at GABAergic Synapses on Parvalbumin-Positive GABAergic Inhibitory Neurons in the Mouse Hippocampus.

The Journal of comparative neurology·2026
Same author

Intercellular communication in the brain through a dendritic nanotubular network.

Science (New York, N.Y.)·2025
Same author

Intercellular communication in the brain via dendritic nanotubular network.

bioRxiv : the preprint server for biology·2025
Same author

Mice with deficiency in Pcdh15, a gene associated with bipolar disorders, exhibit significantly elevated diurnal amplitudes of locomotion and body temperature.

Translational psychiatry·2024
Same author

Large-scale animal model study uncovers altered brain pH and lactate levels as a transdiagnostic endophenotype of neuropsychiatric disorders involving cognitive impairment.

eLife·2024

Related Experiment Video

Updated: Jun 26, 2025

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
04:35

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

Published on: July 26, 2011

16.3K

Development and Application of Technology for Neural Circuit Visualization - Secondary Publication.

Shigeo Okabe1

  • 1Department of Cellular Neurobiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

JMA Journal
|May 9, 2024
PubMed
Summary

Neuronal development involves dynamic cytoskeletal changes and rapid synapse turnover, challenging old theories. This study reveals increased synapse dynamics as a key factor in autism spectrum disorder models.

Keywords:
CytoskeletonGFPLive imagingNeuronSynapse

More Related Videos

Neurovascular Network Explorer 2.0: A Simple Tool for Exploring and Sharing a Database of Optogenetically-evoked Vasomotion in Mouse Cortex In Vivo
08:32

Neurovascular Network Explorer 2.0: A Simple Tool for Exploring and Sharing a Database of Optogenetically-evoked Vasomotion in Mouse Cortex In Vivo

Published on: May 4, 2018

6.4K
Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
09:05

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Published on: July 31, 2017

11.5K

Related Experiment Videos

Last Updated: Jun 26, 2025

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
04:35

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

Published on: July 26, 2011

16.3K
Neurovascular Network Explorer 2.0: A Simple Tool for Exploring and Sharing a Database of Optogenetically-evoked Vasomotion in Mouse Cortex In Vivo
08:32

Neurovascular Network Explorer 2.0: A Simple Tool for Exploring and Sharing a Database of Optogenetically-evoked Vasomotion in Mouse Cortex In Vivo

Published on: May 4, 2018

6.4K
Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
09:05

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Published on: July 31, 2017

11.5K

Area of Science:

  • Neurobiology
  • Cellular Neuroscience
  • Molecular Neuroscience

Background:

  • Neurite extension and synaptic connections are crucial for neural circuits.
  • Traditional models of axonal transport and synapse stability have been challenged by new imaging techniques.

Purpose of the Study:

  • To investigate the dynamic nature of neuronal cytoskeleton and synapses using advanced imaging.
  • To explore the role of synapse dynamics in the pathophysiology of autism spectrum disorders (ASDs).

Main Methods:

  • Utilizing fluorescent dyes for cytoskeletal protein labeling and microinjection into neurons.
  • Employing green fluorescent protein (GFP) tagging for live imaging of synapses and molecular turnover.
  • Quantifying postsynaptic molecules using single GFP molecule fluorescence measurements.
  • Analyzing synapse dynamics in mouse models of ASDs.

Main Results:

  • Axonal elongation is driven by cytoskeleton polymerization at the axon tip, revising slow axonal transport models.
  • Synapses exhibit continuous turnover and rapid molecular composition changes, contradicting theories of stability.
  • Enhanced synapse turnover was identified as a common circuit-level phenotype in mouse models of ASDs.

Conclusions:

  • Neuronal structures like the cytoskeleton and synapses are highly dynamic, not static.
  • Increased synapse dynamics contribute to the pathophysiology of ASDs.
  • Fluorescence imaging is a powerful tool for understanding neuronal dynamics and neural circuit function.