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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

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Two new oleanane triterpenes from Maytenus hookeri.

Quan-Yu Yang¹, Song-Xue Yang¹, Qiong Wei¹

¹Key Laboratory of Medicinal Chemistry for Natural Resource of Ministry of Education, Yunnan Characteristic Plant Extraction Laboratory, Yunnan Research and Development Center for Natural Products, School of Chemical Science and Technology, and School of Pharmacy, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, China.

Journal of Asian Natural Products Research

|May 9, 2024

Summary

Two new triterpenes were discovered from Maytenus hookeri. Compounds 4-7 demonstrated significant inhibitory activity against the NLRP3 inflammasome, a key target in inflammatory diseases.

Keywords:

Celastraceae Maytenus hookeri anti-inflammatory activity chemical composition triterpenes

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Area of Science:

Natural Product Chemistry
Immunology
Pharmacology

Background:

Maytenus hookeri is a plant species known for its diverse chemical constituents.
The NLRP3 inflammasome plays a critical role in inflammatory responses and related diseases.

Purpose of the Study:

To isolate and characterize new compounds from Maytenus hookeri stems.
To evaluate the inhibitory potential of isolated compounds against the NLRP3 inflammasome.

Main Methods:

Isolation of compounds using chromatographic techniques.
Structure elucidation of new compounds via 1D, 2D NMR, HRESIMS, and ECD calculations.
Assessment of NLRP3 inflammasome inhibitory activity using IC50 assays.

Main Results:

Two novel triterpenes, mayteneri A (1) and mayteneri B (2), were identified.
Seven known compounds (3-9) were also isolated and characterized.
Compounds 4-7 exhibited significant NLRP3 inflammasome inhibition, with IC50 values ranging from 2.36 to 3.44 μM.

Conclusions:

Maytenus hookeri is a valuable source of bioactive natural products.
Compounds 4-7 represent promising lead compounds for the development of NLRP3 inflammasome inhibitors.
Further research into these compounds could lead to novel anti-inflammatory therapeutics.