Comparative analysis of the tumor microbiome, molecular profiles, and immune cell abundances by HPV status in mucosal head and neck cancers and their impact on survival
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View abstract on PubMed
Summary
This summary is machine-generated.Specific microbes in Head and Neck Squamous Cell Carcinoma (HNSCC) impact patient survival. Human Papillomavirus (HPV) presence correlates with better outcomes, while certain bacteria like Yersinia pseudotuberculosis are linked to worse survival.
Area Of Science
- Oncology
- Microbiology
- Immunology
Background
- Head and Neck Squamous Cell Carcinoma (HNSCC) exhibits diverse treatment responses and prognoses.
- The tumor microenvironment (TME), including its microbiome and immune cell composition, significantly influences HNSCC patient outcomes.
- Understanding the interplay between microbes, gene expression, and immune infiltration is crucial for predicting survival.
Purpose Of The Study
- To investigate the association between specific microbial presence, gene expression, and tumor immune infiltration in HNSCC.
- To correlate identified microbial signatures and immune profiles with overall survival (OS) in HNSCC patients.
- To leverage The Cancer Genome Atlas (TCGA) data for comprehensive analysis of HNSCC tumor transcriptomics.
Main Methods
- Utilized RNA sequencing (RNAseq) data from 499 HNSCC tumor samples in TCGA.
- Employed the exogenous sequences in tumors and immune cells (exotic) pipeline to detect and quantify low-abundance microbes.
- Assessed Human Papillomavirus (HPV) status by detecting the Papillomaviridae family of viruses and performed statistical analyses using R.
Main Results
- HPV was detected in 22% of samples, predominantly Alphapapillomavirus 9, which was associated with improved OS (HR=0.60, p=0.01).
- Yersinia pseudotuberculosis showed association with the worst survival (HR=3.88, p=0.008), while Pseudomonas viridiflava was linked to the best survival (HR=0.05, p=0.036).
- HPV-negative tumors had higher abundance of gram-negative anaerobes, M0, and M2 macrophages, whereas HPV-positive tumors showed increased T regulatory cells and CD8+ T-cells.
Conclusions
- Specific microbial signatures within HNSCC tumors are significantly associated with patient survival.
- The TME composition, including microbial and immune cell profiles, can serve as a predictive biomarker for HNSCC patient outcomes.
- These findings may elucidate mechanisms of resistance to systemic therapies and guide future treatment strategies.
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