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Phenotyping of proliferating cells in cultures of human lymphocytes
Lymphocytes stimulated with mitogens, alloantigens or soluble antigens were pulsed with tritiated thymidine to label proliferating cells. Aliquots of the cells were labeled with OKT 4 or OKT 8 mouse monoclonal antibodies and then incubated in microtiter wells coated with goat anti-mouse immunoglobulin G (IgG). Unattached cells were flushed out of the wells: individual wells were then separated for counting in scintillation fluid in a B counter. This method permits simple characterization of the relative T cell subset proliferative responses and is clinically applicable.
Lymphocytes stimulated with mitogens, alloantigens or soluble antigens were pulsed with tritiated thymidine to label proliferating cells. Aliquots of the cells were labeled with OKT 4 or OKT 8 mouse monoclonal antibodies and then incubated in microtiter wells coated with goat anti-mouse immunoglobulin G (IgG). Unattached cells were flushed out of the wells: individual wells were then separated for counting in scintillation fluid in a B counter. This method permits simple characterization of the relative T cell subset proliferative responses and is clinically applicable.