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Updated: Jun 26, 2025

Normothermic Negative Pressure Ventilation Ex Situ Lung Perfusion: Evaluation of Lung Function and Metabolism

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Published on: February 14, 2022

Post-mortem Tissue Degassing Using Positive Pressure Is Superior to Negative Pressure.

Victor Barrere¹, Yuanshan Wu², Aiguo Han³

¹Research Service, VA San Diego Healthcare System, San Diego, CA, USA; Department of Orthopaedic Surgery, University of California, San Diego, San Diego, CA, USA.

Ultrasound in Medicine & Biology

|May 9, 2024

Summary

This summary is machine-generated.

Positive pressure effectively reduces gas in biological tissues for acoustic imaging, unlike negative pressure which may paradoxically increase gas. This study highlights positive pressure as a reliable method for tissue degassing.

Keywords:

Degassing Post-mortem imaging Putrefactive gas Quantitative ultrasound

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Area of Science:

Biomedical Engineering
Acoustic Imaging
Tissue Preparation

Background:

Gas inclusions in biological tissues can impede acoustic imaging quality.
Effective tissue degassing is crucial for accurate ultrasound analysis.

Purpose of the Study:

To compare the efficacy of positive pressure (PP) and negative pressure (NP) in reducing gas inclusions in biological tissues.
To determine the optimal method for tissue preparation for acoustic imaging.

Main Methods:

Porcine liver samples were subjected to either PP (0.41 MPa for 10 min) or NP (-0.08 MPa for 30 min) in degassed saline.
Backscatter coefficients (BSCs) were measured before and after treatment to quantify gas reduction.
Paired-samples t-tests were used for statistical analysis.

Main Results:

Positive pressure significantly decreased BSC (p = .001), indicating reduced gas content.
Negative pressure showed no significant change in BSC (p = .177) and, for most samples, increased gas content.
PP resulted in a mean BSC decrease from -13.0 ± 4.3 dB to -18.9 ± 5.0 dB.

Conclusions:

Simple positive pressure chambers effectively reduce tissue gas at lower pressures than previously reported.
Negative pressure (vacuum) methods are ineffective for tissue degassing and may be counterproductive.
Positive pressure is recommended for preparing biological tissues for acoustic imaging to minimize gas inclusions.