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A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
Published on: August 30, 2019
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Revisiting pancreatic islet isolation in murine models: A practical and effective technical protocol
Aline Fernandes-da-Silva1, Rosiane Aparecida Miranda2, Patricia Cristina Lisboa2
1Laboratory of Morphometry, Metabolism, and Cardiovascular Diseases, Biomedical Center, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Physiological Reports
|May 9, 2024
Summary
This study presents a straightforward method for isolating pancreatic islets from mice and rats. This technique aids researchers in studying glycemic homeostasis and hormone secretion in endocrine pancreas research.
Area of Science:
- Endocrinology
- Cell Biology
- Physiology
Background:
- The endocrine pancreas, comprising pancreatic islets, regulates blood glucose via insulin and glucagon secretion.
- Studying islet function requires effective isolation techniques, which can be complex and vary between species.
- Murine models are commonly used for pancreatic research due to practical advantages.
Purpose of the Study:
- To provide a simple and effective protocol for isolating intact pancreatic islets from mice and rats.
- To serve as a practical guide for researchers investigating endocrine pancreatic physiology.
- To facilitate studies on hormone synthesis and secretion.
Main Methods:
- Islet isolation via intraductal collagenase digestion of the pancreatic acinar parenchyma.
- Adaptation of existing rodent islet isolation techniques for broader applicability.
- Focus on a meticulous yet accessible procedural methodology.
Main Results:
- Successful isolation of intact pancreatic islets from both mice and rats.
- The described method is effective and practical for researchers.
- Isolated islets are suitable for various downstream analyses.
Conclusions:
- The developed protocol offers a reliable method for pancreatic islet isolation in rodents.
- This technique supports in vitro endocrine secretion analyses, microscopy, and biochemical studies.
- Facilitates advancements in understanding glycemic homeostasis and pancreatic islet function.
