Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Analysis Of The Diagnostic Performance Of Pax1/sox1 Gene Methylation In Cervical Precancerous Lesions And Its Role In Triage Diagnosis

Analysis of the diagnostic performance of PAX1/SOX1 gene methylation in cervical precancerous lesions and its role in triage diagnosis

Chunli Fan1, Junbo Hu2, Ting Luo3

  • 1Department of Scientific Research and Teaching Management Department, Maternal and Child Health Hospital of Hubei Province, Wuhan, China.

Journal of Medical Virology
|May 10, 2024

Related Experiment Videos

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

5.5K
LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
12:18

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Published on: February 1, 2020

5.8K
Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

8.6K

View abstract on PubMed

Summary
This summary is machine-generated.

This study shows that PAX1/SOX1 gene methylation testing is effective for diagnosing cervical precancerous lesions. This epigenetic marker holds significant value for triage diagnosis in cervical cancer screening.

Area of Science:

  • Epigenetics
  • Oncology
  • Molecular Diagnostics

Background:

  • DNA methylation patterns are crucial epigenetic alterations linked to various diseases, including cancer.
  • Methylation panels offer insights into disease-specific patterns, aiding in diagnosis and prognosis.
  • The diagnostic utility of methylation panels targeting the sex-determining region Y-box 1 (SOX1) and paired box gene 1 (PAX1) for cervical lesions remains under-explored.

Purpose of the Study:

  • To evaluate the diagnostic performance of PAX1/SOX1 gene methylation for detecting cervical precancerous lesions.
  • To assess the potential of PAX1/SOX1 methylation as a triage diagnostic tool.
  • To analyze the sensitivity, specificity, and area under the curve (AUC) of PAX1/SOX1 methylation detection.

Main Methods:

  • A cohort of 181 patients with abnormal HPV-DNA or cytology results underwent colposcopy, cytology, HPV testing, and PAX1/SOX1 methylation detection.
Keywords:
PAX1/SOX1 genesdiagnostic efficacymethylation detectiontriage

Related Experiment Videos

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

5.5K
LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
12:18

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Published on: February 1, 2020

5.8K
Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

8.6K
  • Patients were histopathologically classified into control, cervical intraepithelial neoplasia grades 1-3 (CIN1-3), and cervical cancer (CC) groups.
  • Diagnostic performance metrics including methylation positivity rates, sensitivity, specificity, and AUC were calculated.

    • Main Results:

    • PAX1/SOX1 methylation positivity rates were significantly higher in CIN2 (100%), CIN3 (90%), and CC (100%) groups compared to controls (17.1%) and CIN1 (22.5%).
    • AUC values for PAX1 methylation in diagnosing CIN2+ and CIN3+ were 0.88 and 0.88, respectively. For SOX1, AUC values for CIN2+ and CIN3+ were 0.80 and 0.92, respectively.
    • In HPV16/18-negative patients, methylation detection achieved 100% sensitivity for CIN2+ and CIN3+, with specificities of 83.0% and 81.1%, respectively. The methylation test also showed potential in a triage setting, with a 22.29% colposcopy referral rate among eligible patients.

    Conclusions:

    • PAX1/SOX1 gene methylation detection demonstrates strong diagnostic efficacy for cervical precancerous lesions.
    • This methylation panel is a valuable tool for triage diagnosis in cervical cancer screening programs.
    • Further research can explore integrating this epigenetic marker into routine cervical cancer screening protocols.