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Related Experiment Video

Updated: Jun 26, 2025

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
15:40

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

Published on: January 24, 2011

33.5K

Transfection in Primary Cultured Neuronal Cells.

Katie F M Marwick1, Giles E Hardingham2

  • 1Centre for Discovery Brain Science, University of Edinburgh, Edinburgh, UK. Katie.Marwick@ed.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|May 10, 2024
PubMed
Summary

Lipofection-mediated transfection introduces foreign nucleic acid into rodent neurons. This method helps study N-methyl-D-aspartate receptors (NMDARs) roles in neuronal function.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Transfection is crucial for introducing foreign nucleic acids into eukaryotic cells.
  • Understanding N-methyl-D-aspartate receptors (NMDARs) roles in neurons is vital for neuroscience research.
  • Primary cortical neurons are a key model system for studying neuronal function.

Purpose of the Study:

  • To describe a lipofection-mediated transfection method for introducing cDNA encoding NMDAR subunits into primary rodent cortical neurons.
  • To provide a reliable technique for genetic manipulation of postmitotic neurons.

Main Methods:

  • Lipofection-mediated transfection was employed.
  • Complementary DNA (cDNA) encoding NMDAR subunits was introduced.
  • Postmitotic rodent primary cortical neurons in culture were used.
Keywords:
CultureLipofectionNMDANeuronTransfection

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Related Experiment Videos

Last Updated: Jun 26, 2025

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
15:40

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

Published on: January 24, 2011

33.5K
Calcium Phosphate Transfection of Primary Hippocampal Neurons
08:22

Calcium Phosphate Transfection of Primary Hippocampal Neurons

Published on: November 12, 2013

25.3K
Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol
10:51

Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol

Published on: May 24, 2012

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Main Results:

  • Successful introduction of foreign nucleic acid (cDNA encoding NMDAR subunits) into primary cortical neurons was achieved.
  • The lipofection method proved effective for genetic manipulation of these neurons.

Conclusions:

  • Lipofection-mediated transfection is a viable method for introducing NMDAR subunit cDNA into cultured rodent cortical neurons.
  • This technique facilitates the study of NMDAR function in neuronal systems.