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Updated: Jun 26, 2025
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Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
Published on: January 24, 2011
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Transfection in Primary Cultured Neuronal Cells
Katie F M Marwick1, Giles E Hardingham2
1Centre for Discovery Brain Science, University of Edinburgh, Edinburgh, UK. Katie.Marwick@ed.ac.uk.
Methods in Molecular Biology (Clifton, N.J.)
|May 10, 2024
Abstract:
Transfection allows the introduction of foreign nucleic acid into eukaryotic cells. It is an important tool in understanding the roles of NMDARs in neurons. Here we describe using lipofection-mediated transfection to introduce cDNA encoding NMDAR subunits into postmitotic rodent primary cortical neurons maintained in culture.
