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Interpretive criteria for temocillin disk diffusion susceptibility testing.

P C Fuchs, A L Barry, C Thornsberry

    European Journal of Clinical Microbiology
    |February 1, 1985
    PubMed
    Summary
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    The 60 microgram temocillin disk accurately determined bacterial susceptibility, offering a reliable method for clinical use. This study established optimal disk diffusion criteria for temocillin, aiding antibiotic susceptibility testing.

    Area of Science:

    • Clinical Microbiology
    • Antimicrobial Susceptibility Testing

    Background:

    • Temocillin is a valuable antibiotic for treating infections caused by Gram-negative bacteria.
    • Standardized methods for determining temocillin susceptibility are crucial for effective clinical application.

    Purpose of the Study:

    • To evaluate the accuracy of temocillin disk diffusion tests using different disk concentrations.
    • To establish reliable interpretive criteria for temocillin disk diffusion.

    Main Methods:

    • Broth microdilution and disk diffusion methods were employed.
    • 677 clinical bacterial isolates were tested against temocillin.
    • Disk diffusion tests utilized temocillin disks with concentrations of 30 and 60 micrograms.

    Main Results:

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    • The 60 microgram temocillin disk demonstrated high interpretive accuracy (96.2%).
    • The 30 microgram disk showed only a marginal increase in minor interpretive errors (1.2%).
    • Specific zone diameter breakpoints were defined for both 30 and 60 microgram disks.

    Conclusions:

    • The 60 microgram temocillin disk is a highly accurate tool for bacterial susceptibility testing.
    • Established interpretive criteria facilitate the reliable use of temocillin disk diffusion in clinical settings.