Clonal specificity of concanavalin A-induced cytotoxic T lymphocytes

The specificity of polyclonally induced cytotoxic T lymphocyte (CTL) precursors has been analyzed under clonal conditions. Primary clones of concanavalin A-inducible CTL, if tested on different targets, revealed that they could distinguish between (a) two different allogeneic tumor targets; (b) allogeneic and syngeneic tumor targets, and (c) syngeneic B blasts and tumor targets. No "nonspecific" CTL clones were generated under these culture conditions, and all clones were found to display classical immunological specificity.