Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

191
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
191
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

172
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
172
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

1.0K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
1.0K
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

1.3K
The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
1.3K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

690
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
690
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.0K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Core-to-core overlap enables efficient interchain charge transport beyond crystalline domains in a conjugated polymer: high fill factors in thick organic photovoltaic cells.

Chemical science·2026
Same author

Gas-loading system compatible with ultrafast magic-angle spinning for solid-state nuclear magnetic resonance in gas atmospheres.

Chemical communications (Cambridge, England)·2026
Same author

Microbial biobanking: safeguarding the tiny treasures for sustainable human welfare.

Folia microbiologica·2026
Same author

Insights into the Structural Coexistence of Hexameric States in Microcrystalline Insulin Formulations in the Solid State.

Molecular pharmaceutics·2026
Same author

High-Resolution Proton NMR Spectra of NH Moieties in Solids Enabled by Offset-Tolerant Nitrogen-14 Decoupling via Fast Magic Angle Spinning at 70 kHz.

The journal of physical chemistry letters·2026
Same author

An integrated workflow for the structure elucidation of nanocrystalline powders.

Communications chemistry·2026

Related Experiment Video

Updated: Jun 26, 2025

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

5.6K

High-resolution heteronuclear correlations between spin-1/2 and half-integer quadrupolar nuclei under fast MAS

Manoj Kumar Pandey1, Yusuke Nishiyama2

  • 1Indian Institute of Technology Ropar, Rupnagar, Punjab 140001, India.

Biophysical Chemistry
|May 10, 2024
PubMed
Summary

A new 2D 35Cl/1H T-HMQC-MQMAS method enhances resolution for structural studies. This technique improves the indirect dimension of spin-3/2 35Cl nuclei, offering superior clarity for complex samples.

Keywords:
Fast magic angle spinningHMQCHeteronuclear correlationsMQMASNuclear magnetic resonanceSolid-state NMR

More Related Videos

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.4K
High-Sensitivity Nuclear Magnetic Resonance at Giga-Pascal Pressures: A New Tool for Probing Electronic and Chemical Properties of Condensed Matter under Extreme Conditions
08:42

High-Sensitivity Nuclear Magnetic Resonance at Giga-Pascal Pressures: A New Tool for Probing Electronic and Chemical Properties of Condensed Matter under Extreme Conditions

Published on: October 10, 2014

11.6K

Related Experiment Videos

Last Updated: Jun 26, 2025

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

5.6K
Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.4K
High-Sensitivity Nuclear Magnetic Resonance at Giga-Pascal Pressures: A New Tool for Probing Electronic and Chemical Properties of Condensed Matter under Extreme Conditions
08:42

High-Sensitivity Nuclear Magnetic Resonance at Giga-Pascal Pressures: A New Tool for Probing Electronic and Chemical Properties of Condensed Matter under Extreme Conditions

Published on: October 10, 2014

11.6K

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Materials Science
  • Structural Chemistry

Background:

  • High-resolution structural elucidation is critical for complex samples with multiple sites.
  • Proton-detected solid-state NMR is valuable for studying quadrupolar nuclei like 35Cl.
  • Existing methods may have limitations in spectral resolution for indirect dimensions.

Purpose of the Study:

  • To develop a novel proton-detected 2D 35Cl/1H T-HMQC-MQMAS pulse sequence.
  • To achieve high-resolution in the indirect dimension for 35Cl nuclei coupled to protons.
  • To enhance spectral clarity for structural analysis of challenging materials.

Main Methods:

  • Utilized a TRAPDOR-based heteronuclear multiple quantum coherence (T-HMQC) approach.
  • Employed symmetric-split-t1 multiple-quantum magic angle spinning (MQMAS) with cosine-modulated low-power pulses.
  • Implemented a 2D pulse sequence under fast magic angle spinning (MAS) at 70 kHz.

Main Results:

  • Successfully developed and demonstrated a proton-detected 2D 35Cl/1H T-HMQC-MQMAS sequence.
  • Achieved high resolution in the indirect dimension for spin-3/2 35Cl nuclei.
  • The new sequence showed superior resolution compared to existing double-quantum satellite-transition (DQST) T-HMQC methods.

Conclusions:

  • The developed 2D 35Cl/1H T-HMQC-MQMAS sequence offers significant improvements in spectral resolution.
  • This method is highly effective for structural elucidation of samples containing 35Cl nuclei.
  • The technique provides a powerful new tool for solid-state NMR spectroscopy of quadrupolar nuclei.