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LncRNA FOXD2-AS1 promotes the growth, invasion and migration of OSCC cells by regulating the
Jian Liu1, Yong Zhang2, Jingjing Wu1
1Department of Stomatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, PR China.
Cancer Genetics
|May 10, 2024
Summary
Long non-coding RNA FOXD2-AS1 promotes oral squamous-cell carcinoma (OSCC) growth and metastasis by regulating the miR-185-5p/PLOD1/Akt/mTOR pathway. Inhibiting FOXD2-AS1 suppressed OSCC progression in vitro and in vivo.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long non-coding RNAs (lncRNAs) are implicated in oral squamous-cell carcinoma (OSCC) development.
- The specific roles of lncRNAs, such as FOXD2-AS1, in OSCC invasion and migration remain unclear.
Purpose of the Study:
- To investigate the molecular and cellular mechanisms of FOXD2-AS1 in oral squamous-cell carcinoma (OSCC).
- To elucidate the role of FOXD2-AS1 in OSCC progression, invasion, and migration.
Main Methods:
- Bioinformatic and prognostic analyses identified differential expression of FOXD2-AS1 and PLOD1.
- Cell viability, migration, and invasion assays (CCK-8, Transwell) were performed after FOXD2-AS1 manipulation.
- Western blot, RT-qPCR, and luciferase reporter assays assessed gene/protein expression and regulatory interactions.
- In vivo studies evaluated the effect of FOXD2-AS1 inhibition on tumor growth.
Main Results:
- FOXD2-AS1 and PLOD1 were highly expressed in OSCC and correlated with poor prognosis.
- FOXD2-AS1 suppression reduced OSCC cell viability, migration, and invasion.
- FOXD2-AS1 negatively regulated miR-185-5p, which in turn negatively regulated PLOD1.
- FOXD2-AS1 and PLOD1 influenced the Akt/mTOR signaling pathway, with FOXD2-AS1 promoting tumor growth.
Conclusions:
- FOXD2-AS1 promotes OSCC growth, invasion, and migration.
- The FOXD2-AS1/miR-185-5p/PLOD1/Akt/mTOR axis is a critical pathway in OSCC progression.
- FOXD2-AS1 serves as a potential therapeutic target for oral squamous-cell carcinoma.
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