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Related Experiment Videos

Carbohydrate chains on IgG2b: a requirement for efficient feedback immunosuppression.

B Heyman, M Nose, W O Weigle

    Journal of Immunology (Baltimore, Md. : 1950)
    |June 1, 1985
    PubMed
    Summary

    Carbohydrate chains are crucial for IgG immunosuppressive effects. A modified antibody lacking these chains showed significantly reduced ability to suppress immune responses, highlighting their importance.

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    Area of Science:

    • Immunology
    • Biochemistry
    • Molecular Biology

    Background:

    • The immunosuppressive properties of antibodies are critical for regulating immune responses.
    • The role of glycosylation in antibody function, particularly immunosuppression, requires further elucidation.
    • Murine IgG2b isotype antibodies are known to possess immunosuppressive capabilities.

    Purpose of the Study:

    • To compare the immunosuppressive ability of a glycosylated IgG2b antibody (GORK) with a deglycosylated version (GORK-Tm).
    • To investigate the impact of carbohydrate chains on the immunosuppressive function of IgG antibodies.
    • To confirm the immunosuppressive potential of the IgG2b isotype.

    Main Methods:

    • Generation of a deglycosylated monoclonal IgG2b antibody (GORK-Tm) using tunicamycin treatment.

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  • Comparison of antigen-binding, protein A-binding, complement fixation, and Fc receptor binding capacities of GORK and GORK-Tm.
  • Assessment of the immunosuppressive effect on plaque-forming cell response in vitro.

    • Main Results:

    • GORK-Tm exhibited deficient complement fixation, antibody-dependent cellular cytotoxicity, and Fc receptor binding compared to GORK.
    • While possessing similar antigen-binding capacity, GORK-Tm was significantly less effective in suppressing the plaque-forming cell response.
    • GORK demonstrated potent immunosuppression, reducing the response by up to 95%.

    Conclusions:

    • Antibody glycosylation is essential for mediating the immunosuppressive effects of IgG.
    • The IgG2b isotype, like other murine isotypes, can effectively suppress humoral immune responses when properly glycosylated.