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Liquid nitrogen storage of cultured T lymphocytes
Journal of Immunological Methods
|April 22, 1985
Abstract:
The conditions required for storing and recovering IL-2-dependent T cell clones from liquid nitrogen were investigated. For maximum cell recovery, cultured T lymphocytes were precooled at 4 degrees C for 15 min in medium containing 10% DMSO and 20% FCS before storage in liquid nitrogen. This method allowed adequate time for DMSO to penetrate the cells before freezing.