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Published on: September 19, 2016
Immune response of BALB/c mice infected with two strains of Corynebacterium pseudotuberculosis
Igor Farias Tavares1, Maria da Conceição Aquino de Sá1, José Tadeu Raynal Rocha Filho1
1Federal University of Bahia. Institute of Health Sciences, Salvador, Bahia, 40110-909, Brazil.
Abstract:
This work evaluated aspects of the immune response of BALB/c mice infected with Corynebacterium pseudotuberculosis (T1 and C57). The fifteen BALB/c mice were euthanized after 70 days of infection and morphologically evaluated, also analyzing the innate and adaptive immune responses. The C57 strain induced more pronounced morphological changes than the T1 strain. There was an increase in CD4+ and CD8+ T cells identified during infection with the C57 strain. Cytokines of the inflammatory profile IL-1α and IL-6 and regulatory IL-13 and IL-10 presented significant differences. Cytokines IL-2, IL-4, INF-γ, IL-22, IL-21, and IL-27 did not differ significantly between groups. The obtained results contribute to a better understanding of the type of response and the immunological mechanisms involved during infection with different strains of C. pseudotuberculosis.
Insights
This study compared immune responses in mice infected with two Corynebacterium pseudotuberculosis strains. The C57 strain caused greater changes and altered T cell and cytokine profiles compared to the T1 strain.
Area of Science:
- Immunology
- Microbiology
- Veterinary Science
Background:
- Corynebacterium pseudotuberculosis infections can cause significant disease in animals.
- Understanding the differential immune responses to various strains is crucial for effective treatment and prevention.
Purpose of the Study:
- To evaluate and compare the innate and adaptive immune responses in BALB/c mice infected with two distinct strains of Corynebacterium pseudotuberculosis (T1 and C57).
- To identify specific immunological markers and morphological changes associated with each strain's infection.
Main Methods:
- BALB/c mice were infected with C. pseudotuberculosis (T1 and C57 strains) for 70 days.
- Morphological evaluation, and analysis of innate and adaptive immune responses including T cell populations (CD4+, CD8+) and cytokine profiles (IL-1α, IL-6, IL-13, IL-10, IL-2, IL-4, INF-γ, IL-22, IL-21, IL-27) were performed.
Main Results:
- The C57 strain induced more severe morphological changes compared to the T1 strain.
- Significant increases in CD4+ and CD8+ T cells were observed during C57 strain infection.
- Distinct differences in inflammatory (IL-1α, IL-6) and regulatory (IL-13, IL-10) cytokine levels were noted between the groups.
Conclusions:
- The study highlights differential immune responses elicited by C. pseudotuberculosis strains T1 and C57 in mice.
- Findings contribute to understanding the immunological mechanisms underlying C. pseudotuberculosis infections, differentiating strain-specific effects.
