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Related Experiment Videos

B-cell stimulatory factor 1 activates resting B cells.

E M Rabin, J Ohara, W E Paul

    Proceedings of the National Academy of Sciences of the United States of America
    |May 1, 1985
    PubMed
    Summary

    B-cell stimulatory factor 1 (BSF-1) prepares resting B cells for activation by increasing cell volume and speeding DNA synthesis. This lymphokine acts independently of anti-IgM antibodies, suggesting a key role in B-cell activation.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • B-cell stimulatory factor 1 (BSF-1) is a T-cell lymphokine.
    • BSF-1 and anti-IgM antibodies stimulate resting B cells to enter G1 phase and synthesize DNA.

    Purpose of the Study:

    • To investigate the role of BSF-1 in B-cell activation.
    • To determine if BSF-1 can prepare B cells for activation without anti-IgM antibodies.

    Main Methods:

    • Used EL-4 cell supernatants and purified BSF-1 (HPLC-BSF-1).
    • Pre-cultured resting B cells with BSF-1.
    • Assessed B-cell response to anti-IgM and BSF-1.
    • Measured cell cycle entry, DNA synthesis, and cell volume.
    • Utilized a monoclonal anti-BSF-1 antibody for inhibition studies.

    Main Results:

    • BSF-1 alone, from supernatants or purified, primes resting B cells.
    • Pre-culture with BSF-1 accelerates subsequent B-cell entry into S phase by ~12 hours.
    • BSF-1 significantly increases resting B-cell volume.
    • Monoclonal anti-BSF-1 antibody inhibits these BSF-1-induced effects.

    Conclusions:

    • BSF-1 acts as a B-cell activation factor.
    • BSF-1 prepares B cells for activation independently of anti-IgM.
    • BSF-1 induces significant cellular changes in resting B cells.

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