Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emergency en Bloc Resection of a Ruptured Hemangiosarcoma Anatomically Associated with the Right Retroperitoneal Space, Kidney, and Caudate Hepatic Lobe in a Dog.

Animals : an open access journal from MDPI·2026
Same author

Process of developing basic veterinary clinical performance guidelines based on common clinical manifestations in Korea.

Journal of veterinary science·2026
Same author

Cationic lipid-based nanoparticles for therapeutic delivery in cancer treatment: physicochemical characteristics, therapeutic cargos, and clinical potential.

Applied microscopy·2026
Same author

Multimodal Analgesia Provides Superior Postoperative Pain Control Following Orthopedic Surgery in Small-Breed Dogs.

Animals : an open access journal from MDPI·2026
Same author

Effect of scan body geometric configuration and library design on digital implant impression accuracy.

The journal of advanced prosthodontics·2026
Same author

Integrating Video and Objective Performance Data: Development of VORTEX-SPV for Assessing Robotic Lobectomy Skills.

Innovations (Philadelphia, Pa.)·2026

Related Experiment Video

Updated: Jun 26, 2025

A Reliable and Reproducible Critical-Sized Segmental Femoral Defect Model in Rats Stabilized with a Custom External Fixator
08:20

A Reliable and Reproducible Critical-Sized Segmental Femoral Defect Model in Rats Stabilized with a Custom External Fixator

Published on: March 24, 2019

8.6K

Reconstruction of Segmental Bone Defect in Canine Tibia Model Utilizing Bi-Phasic Scaffold: Pilot Study.

Dae-Won Haam1, Chun-Sik Bae2, Jong-Min Kim3

  • 1Department of Prosthodontics, College of Dentistry, Yonsei University, Seoul 03722, Republic of Korea.

International Journal of Molecular Sciences
|May 11, 2024
PubMed
Summary

A novel synthetic bone graft made of hydroxyapatite and alumina successfully repaired large bone defects in canine tibias. This biomaterial promotes robust bone regeneration and vascularization, offering a promising alternative to current treatments.

Keywords:
canine modelceramichydroxyapatitereconstructionscaffoldsegmental bone defect

More Related Videos

Establishment of a Segmental Femoral Critical-size Defect Model in Mice Stabilized by Plate Osteosynthesis
06:38

Establishment of a Segmental Femoral Critical-size Defect Model in Mice Stabilized by Plate Osteosynthesis

Published on: October 12, 2016

10.1K
Use of Human Perivascular Stem Cells for Bone Regeneration
07:05

Use of Human Perivascular Stem Cells for Bone Regeneration

Published on: May 25, 2012

21.2K

Related Experiment Videos

Last Updated: Jun 26, 2025

A Reliable and Reproducible Critical-Sized Segmental Femoral Defect Model in Rats Stabilized with a Custom External Fixator
08:20

A Reliable and Reproducible Critical-Sized Segmental Femoral Defect Model in Rats Stabilized with a Custom External Fixator

Published on: March 24, 2019

8.6K
Establishment of a Segmental Femoral Critical-size Defect Model in Mice Stabilized by Plate Osteosynthesis
06:38

Establishment of a Segmental Femoral Critical-size Defect Model in Mice Stabilized by Plate Osteosynthesis

Published on: October 12, 2016

10.1K
Use of Human Perivascular Stem Cells for Bone Regeneration
07:05

Use of Human Perivascular Stem Cells for Bone Regeneration

Published on: May 25, 2012

21.2K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Orthopedic Surgery

Background:

  • Large segmental bone defects present significant clinical challenges.
  • Current bone regeneration strategies using scaffolds with cells/growth factors have limitations like immunogenicity and tumorigenesis.
  • The native biogenic micro-environment is critical for bone regeneration.

Purpose of the Study:

  • To develop and evaluate a highly porous, bi-phasic ceramic synthetic bone graft for repairing critical segmental bone defects.
  • To assess the osteogenic and angiogenic potential of the synthetic graft in a canine model.

Main Methods:

  • A synthetic bone graft composed of hydroxyapatite (HA) and alumina (Al) was fabricated with controlled pore sizes (primary, secondary, tertiary) mimicking trabecular bone.
  • A 2 cm critical segmental defect was created in canine tibias and repaired using the synthetic graft.
  • Bone regeneration was assessed using scintigraphy, micro-CT, histology, and dynamic histomorphometry at 8 weeks post-implantation.

Main Results:

  • The synthetic graft exhibited a porosity of 82.61 ± 1.28% with hierarchical pore structures designed for cell support and nutrient diffusion.
  • Micro-CT and histological analyses confirmed robust bone formation and successful reunion of the critical defect.
  • Histology revealed significant vascularization and the formation of both trabecular and cortical bone within the defect area by 8 weeks.

Conclusions:

  • The developed bi-phasic ceramic synthetic bone graft effectively promotes bone regeneration and vascularization in large segmental defects.
  • This synthetic graft offers a promising, potentially off-the-shelf, alternative to existing bone grafting methods, overcoming issues of immunogenicity and tumorigenesis.