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Author Spotlight: Advancing Tendon Tissue Engineering with 3D Organoid Models

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Assessing Bioprinted Functionalized Grafts for Biological Tendon Augmentation In Vitro.

Cristina Del Amo^1,2, Miguel Perez-Garrastachu^1,3, Ines Jauregui²

¹Regenerative Therapies, Biobizkaia Health Research Institute, 48903 Barakaldo, Bizkaia, Spain.

International Journal of Molecular Sciences

|May 11, 2024

Summary

This summary is machine-generated.

Bioprinted tendon grafts using platelet-rich plasma (PRP) activate inflammatory and angiogenic pathways. These PRP grafts influence cell migration and vascularization, offering potential for tendinopathy treatment.

Keywords:

angiogenesis extrusion bioprinting grafts inflammation platelet-rich plasma tendon

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Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Tendinopathy poses significant challenges in sports medicine due to limitations in conservative treatments.
Bioprinting offers a promising approach for developing functional tendon grafts.

Purpose of the Study:

To investigate the paracrine signaling of bioprinted tendon grafts infused with platelet-rich plasma (PRP) in inflamed and non-inflamed environments.
To understand the role of PRP in graft-mediated interactions within host tissues.

Main Methods:

Extrusion bioprinting of tendon cells within PRP-infused methacrylate gelatin (GelMA) hydrogels.
Bioinformatic analysis of overexpressed proteins to predict functional enrichment.
Assessment of graft behavior in both inflamed and non-inflamed host tissues.

Main Results:

PRP grafts activated inflammatory pathways (e.g., IL-17, IL-33) and signaling cascades (MAPK, NF-kB, VEGF).
Enriched biological functions included cell chemotaxis, collagen turnover, cell migration, and angiogenesis.
Acellular PRP grafts promoted increased vessel length, area, and density compared to nude grafts.

Conclusions:

Paracrine signaling from PRP grafts, particularly chemokine activity, modulates cell migration, inflammation, and angiogenesis.
Newly synthesized IL-8, in conjunction with IL-1b, regulates vascularization in cellular grafts under inflammatory conditions.